MAdCAM1 Protein, Human (HEK293, His)

MAdCAM1 Protein is an important cell adhesion molecule and a member of the immunoglobulin superfamily. MAdCAM1 Protein plays a key role in immune system cell migration and mucosal immune responses. MAdCAM1 Protein, Human (HEK293, His) is a recombinant MAdCAM1 protein expressed by HEK293, with a C-6*His tag and glycosylation modification^[1].

MAdCAM1 is a transmembrane glycoprotein expressed by endothelial cells, primarily located in the vascular endothelium of gut-associated lymphoid tissues and blood vessels at chronic inflammatory sites. MAdCAM1 mediates the homing of lymphocytes to intestinal mucosal tissues and organs such as the liver under inflammatory conditions by binding to the integrin α4β7 on the surface of lymphocytes. MAdCAM1 is upregulated during intestinal inflammation or infection to promote lymphocyte infiltration and enhance local immune defense, and its abnormal expression is closely associated with inflammatory bowel disease^[1].

PF-00547659 binds to MAdCAM1 Protein, Human and inhibits the adhesion of α4β7-expressing cells to human and cynomolgus MAdCAM in vitro^[2].

1.Measured by the ability of the immobilized protein to support the adhesion of Jurkat human T-lymphocyte leukemia cells in the presence of 1 mM MnCl2. The ED₅₀ for this effect is 1.987 μg/mL, corresponding to a specific activity is 503.2 units/mg.
2.Measured by the ability of the immobilized protein to support the adhesion of Jurkat human T-lymphocyte leukemia cells. When 5×10⁴ cells/well are added to Human MAdCAM-1 coated plates (2.5 μg/mL with 100 µL/well), approximately 52.575%-62.728% will adhere after 1 hour incubation at 37 °C in the presence of 1 mM MnCl₂.

• 
  Measured by the ability of the immobilized protein to support the adhesion of Jurkat human T-lymphocyte leukemia cells in the presence of 1 mM MnCl2. The ED₅₀ for this effect is 1.987 μg/mL, corresponding to a specific activity is 503.2 units/mg.

Human

HEK293

C-6*His

Q4PKD0 (Q19-Q333)

/

Partial

QSLQVKPLQVEPPEPVVAVALGASRQLTCRLACADRGASVQWRGLDTSLGAVQSDTGRSVLTVRNASLSAAGTRVCVGSCGGRTFQHTVQLLVYAFPDQLTVSPAALVPGDPEVACTAHKVTPVDPNALSFSLLVGGQELEGAQALGPEVQEEEEEPQGDEDVLFRVTERWRLPPLGTPVPPALYCQATMRLPGLELSHRQAIPVLHSPTSPEPPDTTSPESPDTTSPESPDTTSQEPPDTTSQEPPDTTSQEPPDTTSPEPPDKTSPEPAPQQGSTHTPRSPGSTRTRRPEISQAGHTQGKVIPTGSSKPAGDQ

Approximately 55-75 kDa due to the glycosylation.

• 
  Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4 or PBS, pH 7.4, 5% trehalose, 5% mannitol and 0.01% Tween80 or PBS, pH 7.4, 8% trehalose.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Schippers A, et al. MAdCAM-1/α4β7 Integrin-Mediated Lymphocyte/Endothelium Interactions Exacerbate Acute Immune-Mediated Hepatitis in Mice. Cell Mol Gastroenterol Hepatol. 2021;11(4):1227-1250.e1.  [Content Brief]

  [2]. Reinisch W, et al. Targeting Endothelial Ligands: ICAM-1/alicaforsen, MAdCAM-1. J Crohns Colitis. 2018 Aug 22;12(suppl_2):S669-S677.  [Content Brief]