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  4. M1R Protein, Monkeypox virus (HEK293, His)

M1R Protein, Monkeypox virus (HEK293, His) is the recombinant virus-derived M1R protein, expressed by HEK293, with C-His tag.

For research use only. We do not sell to patients.

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Description

M1R Protein, Monkeypox virus (HEK293, His) is the recombinant virus-derived M1R protein, expressed by HEK293, with C-His tag.
Background

M1R is a muscarinic acetylcholine receptor that mediates various cellular responses, primarily through the action of G proteins, including inhibition of adenylate cyclase, breakdown of phosphoinositides, and modulation of potassium channels. Its primary transducing effect is Pi turnover.
Species

Virus
Source

HEK293
Tag

C-8*His
Accession

QJQ40223 (G2-G183)
Protein Length

Partial
Predicted Molecular Mass
20.4 kDa
Molecular Weight

Approximately 23-38 kDa,based on SDS-PAGE under reducing conditions,due to the glycosylation.
Purity

Greater than 95% as determined by reducing SDS-PAGE.
Endotoxin Level

<1 EU/μg, determined by LAL method.
Documentation
Handling Instructions (2659 KB)

M1R Protein, Monkeypox virus (HEK293, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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M1R Protein, Monkeypox virus (HEK293, His)
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