2. Recombinant Proteins
  3. Enzymes & Regulators
  4. Hydrolases (EC 3)
  5. LIPA Protein, Human (P. pastoris, His)

LIPA Protein, Human (P. pastoris, His) is the recombinant human-derived LIPA protein, expressed by P. pastoris, with N-6*His tag.

For research use only. We do not sell to patients.

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Description

LIPA Protein, Human (P. pastoris, His) is the recombinant human-derived LIPA protein, expressed by P. pastoris, with N-6*His tag.
Background

LAL is an enzyme that catalyzes the deacylation of cholesteryl ester core lipids in endocytosed low density lipoproteins to produce free fatty acids and cholesterol. Additionally, it hydrolyzes triglycerides (1,2,3-triacylglycerol) and diglycerides (such as 1,2-diacylglycerol and 1,3-diacylglycerol), with a preference for acyl moieties at the sn-1 or sn-3 positions. by similar: This function has been confirmed in multiple studies.
Species

Human
Source

P. pastoris
Tag

N-6*His
Accession

P38571-1 (S22-Q399)
Gene ID

3988
Protein Length

Partial
Synonyms
Lysosomal acid lipase/cholesteryl ester hydrolase; Cholesteryl esterase; Diacylglycerol lipase; Sterol esterase
AA Sequence

SGGKLTAVDPETNMNVSEIISYWGFPSEEYLVETEDGYILCLNRIPHGRKNHSDKGPKPVVFLQHGLLADSSNWVTNLANSSLGFILADAGFDVWMGNSRGNTWSRKHKTLSVSQDEFWAFSYDEMAKYDLPASINFILNKTGQEQVYYVGHSQGTTIGFIAFSQIPELAKRIKMFFALGPVASVAFCTSPMAKLGRLPDHLIKDLFGDKEFLPQSAFLKWLGTHVCTHVILKELCGNLCFLLCGFNERNLNMSRVDVYTTHSPAGTSVQNMLHWSQAVKFQKFQAFDWGSSAKNYFHYNQSYPPTYNVKDMLVPTAVWSGGHDWLADVYDVNILLTQITNLVFHESIPEWEHLDFIWGLDAPWRLYNKIINLMRKYQ

Predicted Molecular Mass
47.0 kDa
Purity

Greater than 90% as determined by reducing SDS-PAGE.
Endotoxin Level

<1 EU/μg, determined by LAL method.
Documentation
SDS (251 KB)

COA (206 KB)

Handling Instructions (2659 KB)

LIPA Protein, Human (P. pastoris, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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LIPA Protein, Human (P. pastoris, His)
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