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  4. LIF Protein, Cynomolgus, (Biotinylated, HEK293, His, Avi)

LIF Protein, Cynomolgus, (Biotinylated, HEK293, His, Avi)

Cat. No.: HY-P703857
Handling Instructions Technical Support

LIF Protein, Cynomolgus, (Biotinylated, HEK293, His, Avi) is the recombinant cynomolgus/rhesus macaque-derived LIF protein, expressed by HEK293, with C-His & Avi tag.

For research use only. We do not sell to patients.

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Description

LIF Protein, Cynomolgus, (Biotinylated, HEK293, His, Avi) is the recombinant cynomolgus/rhesus macaque-derived LIF protein, expressed by HEK293, with C-His & Avi tag.
Background

The LIF protein possesses the capacity to induce terminal differentiation in leukemic cells. Its diverse range of activities encompasses the induction of hematopoietic differentiation in both normal and myeloid leukemia cells, prompting neuronal cell differentiation, and stimulating acute-phase protein synthesis in hepatocytes. These multifaceted activities underscore LIF's role in influencing cellular differentiation across various cell types and physiological contexts.
Species

Cynomolgus; Rhesus Macaque
Source

HEK293
Tag

C-His;Avi
Accession

A0A2K5UCA6 (S27-F206)
Gene ID

/
Protein Length

Full Length of Mature Protein
Synonyms
leukemia inhibitory factor
Predicted Molecular Mass
23 kDa
Molecular Weight

Approximately 43.7 kDa,based on SDS-PAGE under reducing conditions.
Purity

Greater than 95% as determined by reducing SDS-PAGE.
Endotoxin Level

<1 EU/μg, determined by LAL method.
Documentation
Handling Instructions (2659 KB)

LIF Protein, Cynomolgus, (Biotinylated, HEK293, His, Avi) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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