Lgr5/GPR49 Protein, Human (HEK293, His)

The Lgr5/GPR49 protein is a receptor for R-spondins that enhances the canonical Wnt signaling pathway and marks stem cells in the intestinal epithelium and hair follicles. Lgr5/GPR49 binds R-spondins (RSPO1-4), binds to phosphorylated LRP6 and Frizzled receptors, initiates Wnt signaling and amplifies target gene expression. Lgr5/GPR49 Protein, Human (HEK293, His) is the recombinant human-derived Lgr5/GPR49 protein, expressed by HEK293, with C-His labeled tag.

The Lgr5/GPR49 Protein serves as the receptor for R-spondins, functioning to potentiate the canonical Wnt signaling pathway while concurrently serving as a distinctive stem cell marker in the intestinal epithelium and hair follicle. Upon binding to R-spondins (RSPO1, RSPO2, RSPO3, or RSPO4), Lgr5/GPR49 associates with phosphorylated LRP6 and frizzled receptors activated by extracellular Wnt, initiating the canonical Wnt signaling pathway and amplifying the expression of target genes. In contrast to classical G-protein coupled receptors, Lgr5/GPR49 does not activate heterotrimeric G-proteins to transduce the signal, highlighting its unique regulatory role in Wnt signaling. It plays a crucial role in the development and maintenance of adult intestinal stem cells during postembryonic development and has been identified in a complex with RNF43 and RSPO1. Additionally, Lgr5/GPR49 interacts with other R-spondin ligands, including RSPO2, RSPO3, and RSPO4, emphasizing its diverse participation in cellular signaling pathways.

Human

HEK293

C-His

O75473-1 (G22-R561)

8549

Approximately 65-75 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

Greater than 90% as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized from 0.22 μm filtered solution of PBS, pH7.4 with trehalose as protectant.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.