2. Recombinant Proteins
  3. Enzymes & Regulators
  4. Transferases (EC 2)
  5. KSR2 Protein, Human (sf9, GST)

KSR2 Protein, Human (sf9, GST) is the recombinant human-derived KSR2, expressed by Sf9 insect cells , with GST labeled tag. ,

For research use only. We do not sell to patients.

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Description

KSR2 Protein, Human (sf9, GST) is the recombinant human-derived KSR2, expressed by Sf9 insect cells , with GST labeled tag. ,
Background

KSR2 is a location-regulated scaffold protein that connects MEK to RAF. It exhibits very low protein kinase activity and can phosphorylate multiple Ser and Thr residues of MAP2K1 with minimal efficiency in vitro. Acting as a MAP2K1/MEK1-dependent allosteric activator of BRAF, KSR2 dimerizes with BRAF upon binding to MAP2K1/MEK1, promoting BRAF-mediated phosphorylation of MAP2K1/MEK1. Interaction with BRAF enhances KSR2-mediated phosphorylation of MAP2K1 (in vitro). KSR2 also negatively regulates signaling by blocking MAP3K8 kinase activity and MAP3K8-mediated pathways, while inhibiting MAP3K3-driven activation of ERK, JNK, and NF-kappa-B pathways, including MAP3K3-mediated interleukin-8 production.
Species

Human
Source

Sf9 insect cells
Tag

GST
Accession

Q6VAB6-1 (R554-L950)
Gene ID

283455
Protein Length

Partial
Synonyms
Kinase suppressor of Ras 2; hKSR2
Predicted Molecular Mass
69 kDa
Purity

Greater than 90% as determined by reducing SDS-PAGE
Documentation
Handling Instructions (2659 KB)

KSR2 Protein, Human (sf9, GST) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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KSR2 Protein, Human (sf9, GST)
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