kshB Protein, Mycobacterium tuberculosis (His)

The kshB protein significantly degrades cholesterol by catalyzing the introduction of the 9a-hydroxyl moiety into 1,4-androstadiene-3,17-dione (ADD), resulting in the 9OHADD intermediate. It is spontaneously converted to HSA through the cleavage of the B ring and aromatization of the A ring. kshB Protein, Mycobacterium tuberculosis (His) is the recombinant kshB protein, expressed by E. coli , with N-6*His labeled tag.

The kshB Protein plays a crucial role in the degradation of cholesterol, catalyzing the introduction of a 9a-hydroxyl moiety into 1,4-androstadiene-3,17-dione (ADD) to generate the 9alpha-hydroxy-1,4-androstadiene-3,17-dione (9OHADD) intermediate. This intermediate undergoes spontaneous transformation into 3-hydroxy-9,10-seconandrost-1,3,5(10)-triene-9,17-dione (HSA) through the meta-cleavage of ring B, accompanied by the aromatization of ring A. Additionally, kshB exhibits versatility in substrate utilization, as it can also act on 4-androstene-3,17-dione (AD), 3-oxo-23,24-bisnorcholesta-4-en-22-oate (4-BNC), 3-oxo-23,24-bisnorcholesta-1,4-dien-22-oate (1,4-BNC), 3-oxo-23,24-bisnorcholesta-4-en-22-oyl-coenzyme A thioester (4-BNC-CoA), and 3-oxo-23,24-bisnorcholesta-1,4-dien-22-oyl-coenzyme A thioester (1,4-BNC-CoA) as substrates. This highlights the protein's pivotal role in cholesterol catabolism and its ability to engage in diverse metabolic pathways.

Others

E. coli

N-6*His

P9WJ93 (M1-E358)

887315

Greater than 90% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.