Klotho beta Protein, Mouse (HEK293, His)

Klotho beta protein represses cholesterol 7-alpha-hydroxylase (CYP7A1), a key enzyme in bile acid synthesis. It enhances binding between fibroblast growth factor receptors and fibroblast growth factor 21, and directly interacts with FGF19 and FGF21. This positions Klotho beta as a crucial modulator of metabolic and growth factor signaling pathways. Klotho beta Protein, Mouse (HEK293, His) is the recombinant mouse-derived Klotho beta protein, expressed by HEK293 , with C-10*His labeled tag.

Klotho beta, a multifunctional protein, plays a role in the transcriptional repression of cholesterol 7-alpha-hydroxylase (CYP7A1), a pivotal enzyme in bile acid synthesis. While its glycosidase activity is likely inactive, Klotho beta demonstrates its molecular versatility by enhancing the binding affinity between fibroblast growth factor receptors (FGFR1 and FGFR4) and fibroblast growth factor 21 (FGF21). The protein also engages in direct interactions with FGF19 and FGF21 through its C-terminus, highlighting its involvement in regulatory pathways associated with metabolic processes and growth factor signaling. This intricate network of interactions positions Klotho beta as a key player in modulating crucial physiological functions.

Immobilized Human FGF21 Protein at 2 μg/mL (100μL/well) can bind Mouse Klotho beta. The ED₅₀ for this effect is 291.3 ng/mL.

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  Immobilized Human FGF21 Protein at 2 μg/mL (100μL/well) can bind Mouse Klotho beta. The ED₅₀ for this effect is 291.3 ng/mL.

Mouse

HEK293

C-10*His

Q99N32 (F53-L995)

83379  [NCBI]

Partial

Approximately 125-130 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

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  Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 8% trehalose.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.