KLHDC2 Protein, Human (sf9, His, GST)

KLHDC2 Protein, Human (sf9, His, GST) is the recombinant human-derived KLHDC2, expressed by sf9 insect cells , with His, GST labeled tag. ,

KLHDC2/EloB/EloC serves as the substrate-recognition component of a Cul2-RING (CRL2) E3 ubiquitin-protein ligase complex within the DesCEND (destruction via C-end degrons) pathway. It recognizes C-degrons (typically motifs of less than ten residues) at the extreme C-terminus of target proteins (including full-length, truncated, or proteolytically cleaved forms), triggering their ubiquitination and degradation. The CRL2(KLHDC2) complex specifically targets proteins ending with a diglycine (Gly-Gly) motif, such as truncated SELENOK/SELENOS selenoproteins resulting from failed UGA/Sec decoding, or the N-terminal fragment of USP1 generated by proteolytic cleavage. It may also indirectly repress CREB3-mediated transcription by interfering with CREB3-DNA binding.

Human

Sf9 insect cells

N-His;N-GST

Q9Y2U9-1 (M1-K362)

23588

Partial

Approximately 69 kDa

Greater than 90% as determined by reducing SDS-PAGE

Solution.

Supplied as a 0.22 μm filtered solution of 50 mM HEPES, pH 7.5, 200 mM NaCl, 20% glycerol, 1 mM DTT.

<1 EU/μg, determined by LAL method.

Please use rapid thawing with running water to thaw the protein.

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.