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  4. IRF9 Protein, Human (Sf9, His)

IRF9 Protein, Human (Sf9, His) is the recombinant human-derived IRF9 protein, expressed by E. coli, with His tag.

For research use only. We do not sell to patients.

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Description

IRF9 Protein, Human (Sf9, His) is the recombinant human-derived IRF9 protein, expressed by E. coli, with His tag.
Background

IRF9 is a transcription factor that plays a crucial role in antiviral immunity. It mediates signaling through type I interferons (IFN-alpha and IFN-beta). Upon binding of type I IFNs to cell surface receptors, Jak kinases (TYK2 and JAK1) are activated, leading to tyrosine phosphorylation of STAT1 and STAT2. IRF9/ISGF3G then associates with the phosphorylated STAT1:STAT2 dimer to form the ISGF3 transcription factor complex, which translocates into the nucleus. ISGF3 binds to the interferon-stimulated response element (ISRE) to activate the transcription of interferon-stimulated genes, thereby driving the cell into an antiviral state.
Species

Human
Source

E. coli
Tag

His
Accession

Q00978 (M1-V393)
Gene ID

10379
Protein Length

Full Length
Synonyms
ISGF3G; hIRF9
Predicted Molecular Mass
45.8 kDa
Purity

Greater than 90% as determined by reducing SDS-PAGE
Endotoxin Level

<1 EU/μg, determined by LAL method.
Documentation
Handling Instructions (2659 KB)

IRF9 Protein, Human (Sf9, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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IRF9 Protein, Human (Sf9, His)
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