Integrin alpha 2B beta 3 Protein, Mouse (HEK293, His)

Integrin α-IIb/β-3 is a multifunctional receptor that recognizes multiple ligands and plays a key role in platelet function and hemostasis. It binds fibronectin, fibrinogen, plasminogen, prothrombin, thrombospondin and vitronectin via the RGD sequence, specifically recognizing the HHLGGGAKQAGDV sequence in fibrinogen. Integrin alpha 2B beta 3 Protein, Mouse (HEK293, His) is a recombinant protein dimer complex containing mouse-derived Integrin alpha 2B beta 3 protein, expressed by HEK293 , with C-His labeled tag. Integrin alpha 2B beta 3 Protein, Mouse (HEK293, His), has molecular weight of 90-113 kDa.

The Integrin alpha-IIb/beta-3 protein is a multifaceted receptor with broad ligand recognition capabilities, including binding to fibronectin, fibrinogen, plasminogen, prothrombin, thrombospondin, and vitronectin through the R-G-D sequence. Specifically, it recognizes the H-H-L-G-G-G-A-K-Q-A-G-D-V sequence in the fibrinogen gamma chain. Upon activation, Integrin alpha-IIb/beta-3 facilitates platelet-platelet interactions by binding soluble fibrinogen, leading to rapid platelet aggregation that forms a physical plug on the ruptured endothelial cell surface. Structurally, it forms a heterodimer, with the alpha subunit consisting of a heavy and a light chain linked by a disulfide bond, and the alpha-IIb subunit associates with beta-3. Additionally, Integrin alpha-IIb/beta-3 directly interacts with RNF181, and its cytoplasmic tail region interacts with CIB1, CIB2, CIB3, and CIB4 in a calcium and magnesium-dependent manner. Furthermore, its interaction with PPIA/CYPA is ROS and PPIase activity-dependent and is increased in the presence of thrombin. This diverse range of interactions highlights the integral role of Integrin alpha-IIb/beta-3 in platelet function and hemostasis.

Mouse

HEK293

C-8*His

Q9QUM0 (L32-R988)&O54890 (E26-D717)

16399&16416

90-113 kDa

Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. Normally 8% trehalose is added as protectant before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.