1. Recombinant Proteins
  2. Cytokines and Growth Factors
  3. Interleukin & Receptors
  4. IL-7
  5. IL-7 Protein, Rat

IL-7 Protein regulates T-cell and B-cell development, expansion, and survival, maintaining lymphoid homeostasis.Its interaction with IL7R and CSF2RG receptors is crucial in mediating these effects.IL-7 Protein, Rat is the recombinant rat-derived IL-7 protein, expressed by E.coli , with tag free.

For research use only. We do not sell to patients.

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  • Biological Activity

  • Technical Parameters

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Description

IL-7 Protein regulates T-cell and B-cell development, expansion, and survival, maintaining lymphoid homeostasis.Its interaction with IL7R and CSF2RG receptors is crucial in mediating these effects.IL-7 Protein, Rat is the recombinant rat-derived IL-7 protein, expressed by E.coli , with tag free.

Background

IL-7 Protein is a crucial hematopoietic cytokine that regulates the development, expansion, and survival of both naive and memory T-cells and B-cells, thereby controlling the population of mature lymphocytes and ensuring lymphoid homeostasis. Its interaction with IL7R and CSF2RG receptors plays a significant role in mediating these effects.

Biological Activity

Measured by its binding ability in a functional ELISA. Immobilized Human IL-7 at 2 μg/mL (100 μL/well) on the plate can bind Rat CD127 Protein(HY-P77011). The ED50 for this effect is 2.158 μg/mL.

Species

Rat

Source

E. coli

Tag

Tag Free

Accession

Q91Y32 (D26-I154)

Gene ID
Molecular Construction
N-term
IL-7 (D26-I154)
Accession # Q91Y32
C-term
Protein Length

Full Length of Mature Protein

Synonyms
Interleukin 7; Lymphopoietin-1; PBGF
Predicted Molecular Mass
14.9 kDa
Molecular Weight

Approximately 15 kDa,based on SDS-PAGE under reducing conditions.

Purity

Greater than 90% as determined by reducing SDS-PAGE

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 8% trehalose.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation

IL-7 Protein, Rat Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
IL-7 Protein, Rat
Cat. No.:
HY-P73227
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