IL-17F, Rat (sf9, His)

Rat IL-17F is predicted to have cytokine binding and dimerization activities and is involved in bacterial defense, IL-17-mediated signaling, and regulation of cytokine production. It acts upstream of the inflammatory response, and its homologue to human IL17F has been implicated in chronic mucocutaneous candidiasis. IL-17F, Rat (sf9, His) is the recombinant rat-derived IL-17F, Rat, expressed by E. coli , with N-6*His labeled tag.

IL-17F, Rat is predicted to possess cytokine binding activity, protein dimerization activity, and signaling receptor binding activity. The gene is anticipated to participate in various processes, including the defense response to bacterium, interleukin-17-mediated signaling pathways, and the regulation of cytokine production. It is predicted to act upstream of or within positive regulation of cytokine production involved in the inflammatory response and positive regulation of transcription by RNA polymerase II. The anticipated subcellular location is in the extracellular space. As the ortholog of human IL17F (interleukin 17F), IL-17F, Rat is implicated in chronic mucocutaneous candidiasis. Despite its functional significance, low expression levels have been observed in the reference dataset, suggesting that its regulatory functions may be tightly controlled or context-dependent.

Measured by its ability to induce IL-6 secretion by NIH-3T3 mouse embryonic fibroblast cells. The ED₅₀ for this effect is 45.19 ng/mL, corresponding to a specific activity is 2.212×10⁴ U/mg.

Rat

E. coli

N-6*His

NP_001015011.2 (A28-A161)

301291  [NCBI]

ARRNPKVGLSALQKAGNCPPLEDNSVRVDIRIFNQNQGISVPRDFQNRSSSPWDYNITRDPDRFPSEIAEAQCRHSGCINAQGQEDGSMNSVPIQQEILVLRREPQGCSNSFRLEKMLIKVGCTCVTPIVHHAA

Approximately 18 kDa

Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized from a 0.22 μm filtered solution of 4 mM HCL, pH 2.5.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.