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  6. IL-17F Protein, Human (Biotinylated, HEK293, His-Avi)

IL-17F Protein, Human (Biotinylated, HEK293, His-Avi)

Cat. No.: HY-P78159
Handling Instructions

IL-17F is an inflammatory homodimeric cytokine that induces many proinflammatory cytokines and chemokines. IL-17F also induces antimicrobial peptides. IL-17F plays a protective role in colon cancer development and can be used for the research of autoimmune diseases, infection and cancer. IL-17F Protein, Human (Biotinylated, HEK293, His-Avi) is a biotinylated recombinant human IL-17F protein with His and Avi tags at the C-terminus and is expressed in HEK293 cells. It consists of 133 amino acids (R31-Q163).

For research use only. We do not sell to patients.

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Description

IL-17F is an inflammatory homodimeric cytokine that induces many proinflammatory cytokines and chemokines. IL-17F also induces antimicrobial peptides. IL-17F plays a protective role in colon cancer development and can be used for the research of autoimmune diseases, infection and cancer[1][2]. IL-17F Protein, Human (Biotinylated, HEK293, His-Avi) is a biotinylated recombinant human IL-17F protein with His and Avi tags at the C-terminus and is expressed in HEK293 cells. It consists of 133 amino acids (R31-Q163).

Background

Interleukin-17F (IL-17F) belongs to the IL-17 cytokine family. IL-17F is expressed in activated CD4 T cells, activated monocytes, basophils and mast cells. IL-17F can be produced by differentiated TH17 cells, lamina propria T cells, memory CD4+ T cells, γδ T cells and NKT cells[1].
The human IL-17F shares 55.90% amino acid sequence identity with mouse and 57.14% identity with rat.
IL-17F is an inflammatory cytokine that induces many proinflammatory cytokines and chemokines, including TGF-β, IL-2, ICAM1, GM-CSF, CCL2, CCL7, TSLP, MMP13, IL-6 and CXCL1. IL-17F also induces antimicrobial peptides including hBD-2, S100A7, S100A8 and S100A9 with IL-22 and can synergize with IL-23 in human eosinophils to promote the production of IL-1β and IL-6. IL-17F is a homodimeric cytokine. IL-17F shares the most similarities with IL-17A (50% homology) and can be produced as an IL-17AF heterodimer. IL-17A, IL-17F and IL-17A/F use the same receptor complex: IL-17RA and IL-17RC heterodimer. They trigger qualitatively similar signaling pathways, and IL-17F exhibits the lowest biological activity. IL-17F shows about 100–1000 times lower affinity to the IL-17RA subunit than IL-17A, and does not compete with IL-17A binding to IL-17RA[1][2].
IL-17F plays a protective role in colon cancer development and can be used for the research of autoimmune diseases, infection and cancer[1][3][4].

In Vivo

IL-17F (5 ng/mL; 16-24 h) induces GRO-α, IL-6, and IL-8 secretion in human primary foreskin fibroblast (BJ) cells[5].
IL-17F binds weakly to IL-17RA.Fc with an EC5 > 2 ng/mL, whereas IL-17A binds IL-17RA.Fc with a value of 19 ng/mL, the IL-17F/IL-17A heterodimer has an EC5 of 329 ng/mL[5].
IL-17F (-15 ng/mL; -72 h) increases the cell viability of PC-3 and DU145[6].
IL-17F (1 ng/mL; 24 h) stimulates the malignant phenotypes of PCa cells and activates PI3K/AKT signalling pathway in PCa cells[6].

Species

Human

Source

HEK293

Tag

C-Avi;C-His

Accession

Q96PD4 (R31-Q163)

Gene ID
Molecular Construction
N-term
IL-17F (R31-Q163)
Accession # Q96PD4
His-Avi
C-term
Synonyms
Cytokine ML-1; IL17F; IL-17F; IL24; IL-24; CANDF6; IL-17F; ML1
Molecular Weight

23-28 kDa

Purity

Greater than 95% as determined by Tris-Bis PAGE.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Documentation
References

IL-17F Protein, Human (Biotinylated, HEK293, His-Avi) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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IL-17F Protein, Human (Biotinylated, HEK293, His-Avi)
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HY-P78159
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