IGFBP-4 Protein, Human (HEK293, Fc)

IGFBP-4 protein is an important IGFBP member that prolongs the half-life of insulin-like growth factor (IGF) and modulates its effects on cell cultures by altering the kinetics of interaction with cell surface receptors. It can inhibit or stimulate IGF-induced growth responses, showing specificity for IGF2 but not IGF1. IGFBP-4 Protein, Human (HEK293, Fc) is the recombinant human-derived IGFBP-4 protein, expressed by HEK293, with C-hFc labeled tag.

IGFBP-4 Protein, a member of the insulin-like growth factor-binding proteins (IGFBPs), plays a vital role in extending the half-life of insulin-like growth factors (IGFs) and modulating their effects on cell culture. With the ability to either inhibit or stimulate IGF-induced growth-promoting effects, IGFBP-4 achieves this regulatory function by altering the interaction dynamics between IGFs and their respective cell surface receptors. Notably, IGFBP-4 exhibits a preferential binding affinity for IGF2 over IGF1, highlighting its specificity in modulating the interactions between different IGF isoforms and their receptors. This differential binding capacity underscores the intricate regulatory role of IGFBP-4 in fine-tuning the cellular responses to IGFs, emphasizing its significance in the complex network that governs growth-related signaling pathways.

Human

HEK293

C-hFc

P22692-1 (D22-E258)

3487

Approximately 60-65 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

Greater than 90% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from 0.22 μm filtered solution of PBS, pH7.4 with trehalose as protectant.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.