IDO-2 Protein, Human (His)

The IDO-2 protein plays a key role in cellular processes, catalyzing the initial and rate-limiting steps in the catabolism of the essential amino acid tryptophan in the kynurenine pathway. This enzymatic activity has been confirmed in multiple studies, establishing IDO-2 as a key regulator of tryptophan metabolism with important implications for immune regulation. IDO-2 Protein, Human (His) is the recombinant human-derived IDO-2 protein, expressed by E. coli , with C-6*His labeled tag.

Indoleamine 2,3-dioxygenase 2 (IDO-2) is an enzyme that catalyzes the first and rate-limiting step in the catabolism of the essential amino acid tryptophan along the kynurenine pathway. This pathway is crucial for the regulation of tryptophan metabolism and has implications in immune regulation. IDO-2's activity contributes to immune modulation by limiting tryptophan availability, which, in turn, affects immune cell function. While IDO-2 is involved in immune regulation, it may not play a significant role in tryptophan-related tumoral resistance, as suggested by certain studies.

Measured by its ability to oxidize L-tryptophan to N-formyl-kynurenine. The specific activity is >15 pmol/min/μg.

Human

E. coli

C-6*His

Q6ZQW0-1 (M1-G407)

169355  [NCBI]

Full Length of Isoform-1

MEPHRPNVKTAVPLSLESYHISEEYGFLLPDSLKELPDHYRPWMEIANKLPQLIDAHQLQAHVDKMPLLSCQFLKGHREQRLAHLVLSFLTMGYVWQEGEAQPAEVLPRNLALPFVEVSRNLGLPPILVHSDLVLTNWTKKDPDGFLEIGNLETIISFPGGESLHGFILVTALVEKEAVPGIKALVQATNAILQPNQEALLQALQRLRLSIQDITKTLGQMHDYVDPDIFYAGIRIFLSGWKDNPAMPAGLMYEGVSQEPLKYSGGSAAQSTVLHAFDEFLGIRHSKESGDFLYRMRDYMPPSHKAFIEDIHSAPSLRDYILSSGQDHLLTAYNQCVQALAELRSYHITMVTKYLITAAAKAKHGKPNHLPGPPQALKDRGTGGTAVMSFLKSVRDKTLESILHPRG

Approximately 45 kDa,based on SDS-PAGE under reducing conditions.

Greater than 95% as determined by reducing SDS-PAGE.

Solution.

1.Supplied as a 0.22 μm filtered solution of 20 mM Tris-HCl, 10% Glycerol, 1 mM EDTA, 250 mM NaCl, pH 8.0.
2.Supplied as a 0.22 μm filtered solution in PBS, pH 7.4.
Please refer to the lot-specific COA for specific buffer information.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

<1 EU/μg, determined by LAL method.

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.