ICAM-5 Protein, Mouse (HEK293, His)

ICAM-5 protein, acting as a ligand for LFA-1, plays a crucial role in cell adhesion, specifically facilitating leukocyte binding to target cells. This interaction is integral to immune responses and inflammation, influencing immune cell recruitment and system modulation. ICAM-5's specificity for LFA-1 underscores its involvement in regulating leukocyte adhesion, a fundamental aspect of immune cell behavior and system functionality. ICAM-5 Protein, Mouse (HEK293, His) is the recombinant mouse-derived ICAM-5 protein, expressed by HEK293 , with C-10*His labeled tag and P47R mutation.

ICAM-5 protein holds significance as a ligand for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). This interaction underscores ICAM-5's crucial role in mediating cell adhesion processes, particularly facilitating the binding of leukocytes to target cells. The engagement between ICAM-5 and LFA-1 is integral to immune responses and inflammatory reactions, where proper cell-cell interactions are vital for immune cell recruitment and modulation of immune system functions. The specificity of ICAM-5 for LFA-1 highlights its involvement in regulating leukocyte adhesion, a fundamental aspect of immune cell behavior and immune system functionality.

Measured by its ability of the immobilized protein to support the adhesion of PMA-stimulated Jurkat cells. When 5×10⁴ cells/well are added to ICAM-5 coated plates (25 μg/mL,100 μL/well), 46.215% will adhere after 1 hour at 37°C.

Mouse

HEK293

C-10*His

Q60625 (L31-R828, P47R)

15898

LEPFWADLQPRVALVERGGSLWLNCSTNCPRPERGGLETSLRRNGTQRGLRWLARQLVDIREPETQPVCFFRCARRTLQARGLIRTFQRPDRVELVPLPSWQPVGENFTLSCRVPGAGPRASLTLTLLRGGQELIRRSFVGEPPRARGAMLTARVLARREDHRVNFSCLAELDLRPHGLGLFANSSAPRQLRTFAMPPHSPSLIAPRVLEVDSERPVTCTLDGLFPAPEAGVYLSLGDQRLNPNVTLDGDSLVATATATASAEQEGTKQLMCVVTLGGETRETQENLTVYSFPTPLLTLSEPEAPEGKMVTISCWAGARALVTLEGIPAAVPGQPAELQLNVTKNDDKRGFFCDAALDVDGETLRKNQSSELRVLYAPRLDDLDCPRSWTWPEGPEQTLHCEARGNPEPSVHCARPEGGAVLALGLLGPVTRALAGTYRCTAVNGQGQAVKDVTLTVEYAPALDSVGCPEHITWLEGTEASLSCVAPGVPPPSVSCVRSGKEEVMEGPLRVAREHAGTYRCEAINARGSAAKNVAVTVEYGPSFEELGCPSNWTWVEGSGKLFSCEVDGKPEPRVECVGSEGASEGIVLPLVSSNSGPRNSMTPGNLSPGIYLCNATNRHGSTVKTVVVSAESPPQMDESSCPSHQTWLEGAEATALACSARGRPSPRVHCSREGAARLERLQVSREDAGTYRCVATNAHGTDSRTVTVGVEYRPVVAELAASPPSVRPGGNFTLTCRAEAWPPAQISWRAPPGALNLGLSSNNSTLSVAGAMGSHGGEYECAATNAHGRHARRITVR

Approximately 130-140 kDa due to the glycosylation.

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  Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized from a 0.2 μm filtered solution of 50 mM Tris-Citrate, 300 mM NaCl, pH 7.4, 8% trehalose.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in PBS. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.