HYOU1 Protein, Human (HEK293, His)

The HYOU1 protein plays a crucial role in coordinating cytoprotective mechanisms in response to hypoxia, indicating its importance in cellular stress responses. It involves a potential molecular chaperone function and is actively involved in the protein folding process. HYOU1 Protein, Human (HEK293, His) is the recombinant human-derived HYOU1 protein, expressed by HEK293 , with C-10*His labeled tag.

The HYOU1 protein is intricately involved in vital cytoprotective responses activated during oxygen deprivation, emphasizing its crucial role in cellular adaptation to hypoxic conditions. Additionally, HYOU1 may function as a molecular chaperone, contributing to protein folding processes. It is a component of a substantial chaperone multiprotein complex that includes DNAJB11, HSP90B1, HSPA5, PDIA2, PDIA4, PDIA6, PPIB, SDF2L1, UGGT1, and minimal amounts of ERP29, while CALR and CANX are either absent or present at very low levels within this complex. These findings highlight the significance of HYOU1 in orchestrating cellular responses to oxygen deprivation and its potential involvement in protein quality control mechanisms.

Measured by ATPase activity using a Malachite Green assay. The specific activity is 1633.88 pmol/min/μg.

Human

HEK293

C-10*His

Q9Y4L1-1 (M695-L999)

10525  [NCBI]

MVEEIGVELVVLDLPDLPEDKLAQSVQKLQDLTLRDLEKQEREKAANSLEAFIFETQDKLYQPEYQEVSTEEQREEISGKLSAASTWLEDEGVGATTVMLKEKLAELRKLCQGLFFRVEERKKWPERLSALDNLLNHSSMFLKGARLIPEMDQIFTEVEMTTLEKVINETWAWKNATLAEQAKLPATEKPVLLSKDIEAKMMALDREVQYLLNKAKFTKPRPRPKDKNGTRAEPPLNASASDQGEKVIPPAGQTEDAEPISEPEKVETGSEPGDTEPLELGGPGAEPEQKEQSTGQKRPLKNDEL

Approximately 53-75 kDa, due to the glycosylation.

Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US;may vary elsewhere.