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  5. Hyaluronidase 2/HYAL2 Protein, Mouse (HEK293, His)

Hyaluronidase 2/HYAL2 Protein, Mouse (HEK293, His)

Cat. No.: HY-P703763
Handling Instructions Technical Support

Hyaluronidase 2/HYAL2 Protein, Mouse (HEK293, His) is the recombinant mouse-derived Hyaluronidase 2/HYAL2 protein, expressed by HEK293, with C-His tag.

For research use only. We do not sell to patients.

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Description

Hyaluronidase 2/HYAL2 Protein, Mouse (HEK293, His) is the recombinant mouse-derived Hyaluronidase 2/HYAL2 protein, expressed by HEK293, with C-His tag.
Background

Hyaluronidase 2 (HYAL2) protein is involved in the hydrolysis of high molecular weight hyaluronic acid, generating intermediate-sized products that are subsequently processed by sperm hyaluronidase to produce small oligosaccharides. Although HYAL2 exhibits very low levels of activity, its role in the breakdown of hyaluronic acid suggests a potential contribution to the modulation of extracellular matrix dynamics. Additionally, HYAL2 associates with and negatively regulates MST1R, indicating a potential regulatory role in cellular processes mediated by MST1R.
Species

Mouse
Source

HEK293
Tag

C-8*His
Accession

O35632 (L23-D448)
Protein Length

Full Length of Mature Protein
Synonyms
Hyaluronidase-2; Hyaluronoglucosaminidase-2; Hyal2; Hyal-2
Predicted Molecular Mass
49.9 kDa
Molecular Weight

Approximately 52-68 kDa,based on SDS-PAGE under reducing conditions,due to the glycosylation.
Glycosylation
Yes
Purity

Greater than 95% as determined by reducing SDS-PAGE.
Endotoxin Level

<1 EU/μg, determined by LAL method.
Documentation
Handling Instructions (2659 KB)

Hyaluronidase 2/HYAL2 Protein, Mouse (HEK293, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Hyaluronidase 2/HYAL2 Protein, Mouse (HEK293, His)
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