HSP90b Protein, Human (sf9)

HSP90b Protein, Human (sf9) is the recombinant human-derived HSP90b, expressed by sf9 insect cells , with tag Free labeled tag.

Hsp90 is a molecular chaperone that facilitates the maturation, structural maintenance, and proper regulation of specific target proteins involved in processes such as cell cycle control and signal transduction. Its functional cycle is linked to ATPase activity, inducing conformational changes in client proteins to activate them. Hsp90 dynamically interacts with various co-chaperones, which modulate substrate recognition, ATPase cycling, and chaperone function. It engages with diverse client protein classes through co-chaperone adapters, forming functional chaperone complexes. After completing the chaperoning process, properly folded clients and co-chaperones dissociate, leaving Hsp90 in an ADP-bound partially open state before ADP release resets the cycle. Beyond chaperoning, Hsp90 regulates transcription by modulating transcription factor levels, epigenetic modifiers (e.g., histone deacetylases or DNA methyltransferases), and histone eviction from gene promoters. It antagonizes STUB1-mediated inhibition of TGF-beta signaling, promotes cell differentiation, and supports STAT1 phosphorylation/activation. Hsp90 also mediates ERGIC translocation of leaderless cargos (e.g., IL-1) via the cargo receptor TMED10. During microbial infection, it binds N. meningitidis NadA, potentially interfering with host cell invasion.

Human

Sf9 insect cells

Tag Free

P08238 (M1-D724)

3326

Full Length

Greater than 95% as determined by reducing SDS-PAGE

Solution.

Supplied as a 0.22 μm filtered solution of 50 mM Tris-HCl, pH 8.0, 200 mM NaCl, 20% glycerol, 1 mM DTT.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

<1 EU/μg, determined by LAL method.

Please use rapid thawing with running water to thaw the protein.

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.