2. Recombinant Proteins
  3. Enzymes & Regulators
  4. Phosphatase
  5. Alkaline Phosphatase
  6. HRP-Labeled Alkaline Phosphatase/ALPL Protein, Human (HEK293, His)

HRP-Labeled Alkaline Phosphatase/ALPL Protein, Human (HEK293, His)

Cat. No.: HY-P704350
Handling Instructions Technical Support

For research use only. We do not sell to patients.

Surface Plasmon Resonance (SPR) Assay Service   Protein Expression Service

Size Stock
50 μg   Get quote  
100 μg   Get quote  

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Top Publications Citing Use of Products

  • Technical Parameters

  • Properties

  • Documentation

Species

Human
Source

HEK293
Tag

C-His
Accession

P05186-1 (L18-S502)
Gene ID

249
Molecular Construction
N-term
ALPL (L18-S502)
Accession # P05186
His
C-term
Protein Length

Partial
Synonyms
rHuAlkaline phosphatase, tissue-nonspecific isozyme/ALPL, His; Alkaline Phosphatase; Tissue-Nonspecific Isozyme; AP-TNAP; TNSALP; Alkaline Phosphatase Liver/Bone/Kidney Isozyme; ALPL
Predicted Molecular Mass
55.3 kDa.
Molecular Weight

40-43 kDa,60-65 kDa and >180 kDa
Glycosylation
Yes
Purity

Greater than 90% as determined by reducing SDS-PAGE.
Endotoxin Level

<1 EU/μg, determined by LAL method.
Documentation
Handling Instructions (2659 KB)

HRP-Labeled Alkaline Phosphatase/ALPL Protein, Human (HEK293, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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HRP-Labeled Alkaline Phosphatase/ALPL Protein, Human (HEK293, His)
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