HPD/HPPDase Protein, Human (His)

HPD is a key enzyme in tyrosine catabolism and plays a key role in catalyzing the conversion of 4-hydroxyphenylpyruvate into homogentisate. This enzymatic process represents a key step in the tyrosine metabolic pathway and contributes to the breakdown of this amino acid. HPD/HPPDase Protein, Human (His) is the recombinant human-derived HPD/HPPDase protein, expressed by E. coli , with N-6*His labeled tag.

HPD, also known as 4-hydroxyphenylpyruvate dioxygenase or HPPDase, is a crucial enzyme in tyrosine catabolism. It plays a pivotal role in the conversion of 4-hydroxyphenylpyruvic acid to homogentisic acid, representing a key step in the degradation pathway of tyrosine. Through its catalytic activity, HPD facilitates the transformation of 4-hydroxyphenylpyruvic acid into homogentisic acid, contributing to the breakdown of tyrosine and the subsequent metabolism of aromatic amino acids. This enzymatic process is essential for maintaining the balance of tyrosine-derived metabolites and is integral to various physiological processes within the cell.

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Human

E. coli

N-6*His

P32754-1 (M1-M393)

3242  [NCBI]

Full Length of Isoform-1

MTTYSDKGAKPERGRFLHFHSVTFWVGNAKQAASFYCSKMGFEPLAYRGLETGSREVVSHVIKQGKIVFVLSSALNPWNKEMGDHLVKHGDGVKDIAFEVEDCDYIVQKARERGAKIMREPWVEQDKFGKVKFAVLQTYGDTTHTLVEKMNYIGQFLPGYEAPAFMDPLLPKLPKCSLEMIDHIVGNQPDQEMVSASEWYLKNLQFHRFWSVDDTQVHTEYSSLRSIVVANYEESIKMPINEPAPGKKKSQIQEYVDYNGGAGVQHIALKTEDIITAIRHLRERGLEFLSVPSTYYKQLREKLKTAKIKVKENIDALEELKILVDYDEKGYLLQIFTKPVQDRPTLFLEVIQRHNHQGFGAGNFNSLFKAFEEEQNLRGNLTNMETNGVVPGM

40-50 kDa

Greater than 95% as determined by reducing SDS-PAGE

Solution.

Supplied as a 0.22 μm filtered solution of 20 mM Tris-HCl, 50 mM NaCl, 1 mM DTT, 20% Glycerol, pH 8.0.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.