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  3. PE-Labeled HLA-G&B2M&Peptide (RIIPRHLQL) Tetramer Protein, Human (HEK293, His-Avi)

PE-Labeled HLA-G&B2M&Peptide (RIIPRHLQL) Tetramer Protein, Human (HEK293, His-Avi)

Cat. No.: HY-P701002
Handling Instructions Technical Support

For research use only. We do not sell to patients.

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Top Publications Citing Use of Products
  • Biological Activity

  • Technical Parameters

  • Properties

  • Documentation

Biological Activity

Immobilized PE-Labeled Human HLA-G&B2M&Peptide (RIIPRHLQL) Tetramer, His Tag at 0.5 μg/mL (100 μl/well) on the plate. Dose response curve for Anti-HLA-G Antibody, hFc Tag with the EC50 of ≤9.2 ng/mL determined by ELISA.

Species

Human

Source

HEK293

Tag

C-Avi;C-8*His

Accession

P17693 (G25-T305)&P61769 (I21-M119)&RIIPRHLQL

Gene ID

3135&567

Synonyms
HLA G antigen; sHLA-G; b2 microglobulin; HLA G; HLAG; HLA-G; MHC Class I Antigen G; MHC class Ib antigen; MHC-G; sHLA-G
Molecular Weight

260-265 kDa under Non reducing (N) condi

Glycosylation
Yes
Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.22 μm filtered solution of PBS, 0.2% BSA, pH 7.4, 8% trehalose.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 1 year from date of receipt, protect from light. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation

PE-Labeled HLA-G&B2M&Peptide (RIIPRHLQL) Tetramer Protein, Human (HEK293, His-Avi) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
PE-Labeled HLA-G&B2M&Peptide (RIIPRHLQL) Tetramer Protein, Human (HEK293, His-Avi)
Cat. No.:
HY-P701002
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