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  4. HLA-A*02:01&B2M&NY-ESO-1(SLLMWITQC) Tetramer Protein, Human (HEK293, His-Avi)

HLA-A*02:01&B2M&NY-ESO-1(SLLMWITQC) Tetramer Protein, Human (HEK293, His-Avi)

Cat. No.: HY-P77757
COA
SDS
Handling Instructions Technical Support

For research use only. We do not sell to patients.

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Top Publications Citing Use of Products

  • Biological Activity

  • Technical Parameters

  • Properties

  • Documentation

Biological Activity

Immobilized Human NY-ESO-1 (HLA-A*02:01) Complex Tetramer at 5 μg/mL (100μL/Well) on the plate. Dose response curve for Anti-NY-ESO-1(HLA-A*02:01) Antibody with the EC50 of 5.2-7 ng/mL determined by ELISA.
Species

Human
Source

HEK293
Tag

C-Avi;C-8*His
Accession

A0A140T913 (G25-T305)&P61769 (I21-M119)&SLLMWITQC
Gene ID

/&567  [NCBI]
Synonyms
MHC; MY-ESO-1; CT6.1; LAGE-2; CTAG1; CTAG1B; ESO1CTAG; LAGE2A; NY-ESO-1
Molecular Weight

260-265 kDa
Glycosylation
Yes
Purity

Greater than 95% as determined by Tris-Bis PAGE.
Appearance

Solution.
Formulation

Supplied as a 0.22 μm filtered solution of PBS, pH 7.4.
Endotoxin Level

<1 EU/μg, determined by LAL method.
Reconstitution

N/A.
Storage & Stability

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping

Shipping with dry ice.
Documentation
SDS (251 KB)

COA (233 KB)

Handling Instructions (2659 KB)

HLA-A*02:01&B2M&NY-ESO-1(SLLMWITQC) Tetramer Protein, Human (HEK293, His-Avi) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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HLA-A*02:01&B2M&NY-ESO-1(SLLMWITQC) Tetramer Protein, Human (HEK293, His-Avi)
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