Hemagglutinin glycoprotein Protein, Measles virus (Cell-Free, His)

The Hemagglutinin glycoprotein initiates infection by binding the virus to cell receptors, inducing a conformational change for fusion triggered by the F protein. It may use CD46 and/or SLAMF1 receptors for entry, leading to down-regulation of CD46, enhancing susceptibility to complement lysis. H protein also interacts with NECTIN4, facilitating viral infection in the respiratory epithelium. Hemagglutinin glycoprotein Protein, Measles virus (Cell-Free, His) is the recombinant Virus-derived Hemagglutinin glycoprotein protein, expressed by E. coli Cell-free , with N-10*His labeled tag.

The Hemagglutinin glycoprotein plays a crucial role in the initiation of infection by attaching the virus to cell receptors. Upon binding to the receptor, the H protein induces a conformational change that facilitates the triggering of fusion between virion and cell membranes by the F protein. The virus may utilize human CD46 and/or SLAMF1 as receptors for cellular entry. The strong interaction between H and MCP/CD46 leads to the down-regulation of the latter from the surface of infected cells, enhancing their susceptibility to c3b-mediated complement lysis. Additionally, the H protein interacts with human NECTIN4, enabling viral infection of the respiratory epithelium.

Virus

E. coli Cell-free

N-10*His

P35971 (M1-R617)

/

Full Length

MSPQRDRINAFYKDNPHPKGSRIVINREHLMIDRPYVLLAVLFVMFLSLIGLLAIAGIRLHRAAIYTAEIHKSLSTNLDVTNSIEHQVKDVLTPLFKIIGDEVGLRTPQRFTDLVKFISDKIKFLNPDREYDFRDLTWCINPPERIKLDYDQYCADVAAEELMNALVNSTLLETRTTNQFLAVSKGNCSGPTTIRGQFSNMSLSLLDLYLGRGYNVSSIVTMTSQGMYGGTYLVEKPNLSSKRSELSQLSMYRVFEVGVIRNPGLGAPVFHMTNYLEQPVSNDLSNCMVALGELKLAALCHREDSITIPYQGSGKGVSFQLVKLGVWKSPTDMQSWVTLSTDDPVIDRLYLSSHRGVIADNQAKWAVPTTRTDDKLRMETCFQQACKGKIQALCENPEWAPLKDNRIPSYGVLSVDLSLTVELKIKIASGFGPLITHGSGMDLYKSNHNNVYWLTIPPMKNLALGVINTLEWIPRFKVSPYLFNVPIKEAGEDCHAPTYLPAEVDGDVKLSSNLVILPGQDLQYVLATYDTSRVEHAVVYYVYSPSRSFSYFYPFRLPIKGVPIELQVECFTWDQKLWCRHFCVLADSESGGHITHSGMVGMGVSCTVTREDGTNRR

75 kDa

Greater than 90% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.22 μm filtered solution of PBS, 0.05% Brij78,6% trehalose, pH7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.