2. Recombinant Proteins
  3. Enzymes & Regulators
  4. Oxidoreductases (EC 1)
  5. 7α‐HSDH Protein, E.coli (His, Strep)

7α‐HSDH Protein, E.coli (His, Strep) is the recombinant E.coli-derived 7α‐HSDH protein, expressed by E. coli, with Strep & His tag.

For research use only. We do not sell to patients.

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Description

7α‐HSDH Protein, E.coli (His, Strep) is the recombinant E.coli-derived 7α‐HSDH protein, expressed by E. coli, with Strep & His tag.
Background

7α-HSDH is a 7alpha-hydroxysteroid dehydrogenase involved in bile acid metabolism. It catalyzes the NAD(+)-dependent oxidation of the 7alpha-hydroxy group of 7alpha-hydroxysteroids, such as the major human bile acids cholate and chenodeoxycholate, to the corresponding 7-oxosteroids. It can also act, though less efficiently, on taurochenodeoxycholate, taurocholate, and glycocholate. By similar function, it can use glycochenodeoxycholate as a substrate but cannot utilize NADP(+) instead of NAD(+) as the electron acceptor.
Species

E.coli
Source

E. coli
Tag

Strep;His
Accession

P0AET8 (F2-N255)
Gene ID

75171679
Protein Length

Partial
Synonyms
hsdH
Predicted Molecular Mass
29.8 kDa
Purity

Greater than 90% as determined by reducing SDS-PAGE
Endotoxin Level

<1 EU/μg, determined by LAL method.
Documentation
Handling Instructions (2659 KB)

7α‐HSDH Protein, E.coli (His, Strep) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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