HCoV-HKU1 Spike Trimer Protein (1264a.a, HEK293, His)

The HCoV-HKU1 spike protein initiates viral infection by binding to host receptors, promoting attachment of viral particles to the cell membrane. As a class I viral fusion protein, it coordinates the fusion between the virion and the cell membrane through a series of conformational states. HCoV-HKU1 Spike Trimer Protein (1264a.a, HEK293, His) is the recombinant virus-derived HCoV-HKU1 Spike Trimer protein, expressed by HEK293, with C-His labeled tag.

The HCoV-HKU1 Spike/S1 Protein plays a pivotal role in the viral infection process by attaching the virion to the cell membrane through interaction with host receptors, thereby initiating infection. Functioning as a class I viral fusion protein, it mediates the fusion of the virion with cellular membranes. The protein undergoes at least three conformational states: a pre-fusion native state, a pre-hairpin intermediate state, and a post-fusion hairpin state. During the fusion process between viral and target cell membranes, the coiled coil regions (heptad repeats) adopt a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain. This structural rearrangement is crucial for driving the apposition and subsequent fusion of viral and target cell membranes.

Virus

HEK293

C-His

Q0ZME7 (A13-N1276)

/

Approximately 180-210 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from 0.22 μm filtered solution in 0.1 M Sodium citrate, pH5.5 with trehalose as protectant.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.