GUCY2C/Guanylyl cyclase C Protein, Human (Biotinylated, HEK293, Fc-Avi)

GUCY2C, a guanylyl cyclase, catalyzes cGMP synthesis from GTP and acts as a receptor for E. coli heat-stable enterotoxin, stimulating cGMP accumulation in expressing mammalian cells. Additionally, endogenous peptides guanylin and uroguanylin activate GUCY2C. This multifaceted role underscores intricate regulatory mechanisms in cellular signaling and GUCY2C's responsiveness to various stimuli, as confirmed by studies. GUCY2C/Guanylyl cyclase C Protein, Human (Biotinylated, HEK293, Fc-Avi) is the recombinant human-derived GUCY2C/Guanylyl cyclase C protein, expressed by HEK293 , with C-Avi, C-hFc labeled tag. The total length of GUCY2C/Guanylyl cyclase C Protein, Human (Biotinylated, HEK293, Fc-Avi) is 407 a.a., with molecular weight of 90-110 kDa.

GUCY2C, a guanylyl cyclase, serves as a catalyst in the synthesis of cyclic (cGMP) from GTP, as evidenced by studies. Additionally, this protein functions as a receptor for the E. coli heat-stable enterotoxin, where the enterotoxin significantly stimulates the accumulation of cin mammalian cells expressing GUCY2C. Furthermore, GUCY2C is activated by endogenous peptides, guanylin and uroguanylin. This multifaceted role underscores the intricate regulatory mechanisms involving GUCY2C in cellular signaling and its responsiveness to various stimuli.

Immobilized Monoclonal Anti-Human GUCY2C Antibody Human IgG1 at 1 μg/mL (100 μL/well) can bind Biotinylated Human GUCY2C Fc-Avi with a linear range of 0.8-25 ng/mL.

Human

HEK293

C-Avi;C-hFc

P25092 (S24-Q430)

2984  [NCBI]

90-110 kDa

Greater than 90% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized a 0.22 μm filtered solution of PBS, pH 7.4. Normally trehalose is added as protectant before lyophilization.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.