GSDMD Protein, Human (His)

GSDMD Protein, Human (His) is the recombinant human-derived GSDMD protein, expressed by E. coli, with N-6*His tag.

Cleaved N-terminal GSDMD is a precursor of a pore-forming protein that plays a key role in host defense against pathogen infection and danger signals. Upon cleavage, the released N-terminal moiety (Gasdermin-D, N-terminal) binds to membranes and forms pores, triggering pyroptosis. It is produced by the cleavage of gasdermin-D by inflammatory caspases (CASP1, CASP4, or CASP5) in response to canonical or non-canonical (e.g., cytosolic LPS) inflammasome activators. After cleavage, it moves to the plasma membrane, strongly binds to inner leaflet lipids (e.g., monophosphorylated phosphatidylinositols, bisphosphorylated phosphatidylinositols), and forms pores of 10-15 nm in diameter, releasing mature interleukin-1 (IL1B and IL18) and inducing pyroptosis. Gasdermin pores also allow the release of mature caspase-7 (CASP7) (by similar). In some cell types, pyroptosis is followed by pyroptotic cell death mediated by downstream ninjurin-1 (NINJ1), causing membrane rupture (cytolysis). It can also form pores in the mitochondrial membrane, releasing mitochondrial DNA (mtDNA) into the cytosol (by similar). The extracellularly released Gasdermin-D, N-terminal binds to and kills Gram-negative and Gram-positive bacteria without harming mammalian cells due to lipid-binding specificity. It is also active against intracellular bacteria (e.g., Listeria monocytogenes) under cell culture conditions (by similar). It is activated in response to Yersinia toxin YopJ-mediated inactivation of MAP3K7/TAK1, leading to cleavage by CASP8 (by similar), and is involved in mucosal defense against enteric pathogens (by similar). Excessive activation of the non-canonical inflammasome in brain endothelial cells can lead to pyroptosis and blood-brain barrier breakdown (by similar). Additionally, it is cleaved by CASP3 or CASP7 in the upper small intestine, acting as a transcription coactivator to maintain food tolerance (by similar). It is also cleaved by the papain allergen, forming pores and specifically releasing mature interleukin-33 (IL33) to promote type 2 inflammatory immune responses.

Human

E. coli

N-6*His

P57764 (M1-H484)

79792

Full Length

57 kDa

Greater than 90% as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized from a 0.22 μm filtered solution of PBS, 6% Trehalose, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.