GRPR Protein, Human (Cell-free, Twin-Strep)

GRPR Protein, Human (Cell-free, Twin-Strep) is the recombinant human-derived GRPR, expressed by E. coli Cell-free , with C-Twin-Strep labeled tag.

GRPR is a receptor for gastrin-releasing peptide (GRP), signaling via G protein-coupled activation of a phosphatidylinositol-calcium second messenger system to induce Akt phosphorylation. It regulates food intake and mediates nonhistaminergic itch sensation in the spinal cord (without pain perception). By similarity, GRPR acts in the amygdala to inhibit fear-associated memory and disinhibits auditory cortex glutamatergic cells via vasoactive intestinal peptide-expressing cells to enhance auditory fear memory. Additionally, it contributes to sigh induction through signaling in the pre-Bötzinger complex, a respiratory rhythm-generating neuronal cluster.

Human

E. coli Cell-free

C-Twin-Strep

P30550 (M1-V384)

2925

Full Length

40 kDa&80 kDa&160 kDa

Greater than 90% as determined by reducing SDS-PAGE

Lyophilized powder.

Lyophilized from a 0.22 μm filtered solution of PBS, 0.05% Brij-78, 6%trehalose, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.