Gremlin-1 Protein, Human (HEK293, His)

Gremlin-1 Protein, Human (HEK293, His) is the recombinant human-derived Gremlin-1 protein, expressed by HEK293, with C-His tag.

Gremlin-1 is a cytokine potentially involved in carcinogenesis and metanephric kidney organogenesis. Functioning as a BMP antagonist, it is essential for early limb outgrowth and patterning by maintaining the FGF4-SHH feedback loop. It downregulates BMP4 signaling in a dose-dependent manner (By similarity). As an antagonist of BMP2, it inhibits BMP2-mediated osteoblast differentiation (in vitro). Additionally, it acts as an inhibitor of monocyte chemotaxis. Overexpression can suppress the growth/viability of normal cells but not transformed cells (By similarity).

Measured by its binding ability in a functional ELISA.Immobilized Human BMP-2, Tag Free at 2 μg/mL (100 μL/well) can bind Human Gremlin, His Tag,the ED₅₀ is determined as 0.017 μg/mL.

Human

HEK293

C-His

NP_037504 (L25-D184)

26585

Full Length of Mature Protein

Approximately 28-35 kDa,based on SDS-PAGE under reducing conditions,due to the glycosylation.

Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized from a 0.22 μm filtered solution of 10 mM Sodium Citrate, pH3.0 with 10% trehalose as protectant.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.