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  4. Gremlin-1 Protein, Human (HEK293, His)

Gremlin-1 Protein, Human (HEK293, His) is the recombinant human-derived Gremlin-1 protein, expressed by HEK293, with C-His tag.

For research use only. We do not sell to patients.

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Description

Gremlin-1 Protein, Human (HEK293, His) is the recombinant human-derived Gremlin-1 protein, expressed by HEK293, with C-His tag.
Background

Gremlin-1 is a cytokine potentially involved in carcinogenesis and metanephric kidney organogenesis. Functioning as a BMP antagonist, it is essential for early limb outgrowth and patterning by maintaining the FGF4-SHH feedback loop. It downregulates BMP4 signaling in a dose-dependent manner (By similarity). As an antagonist of BMP2, it inhibits BMP2-mediated osteoblast differentiation (in vitro). Additionally, it acts as an inhibitor of monocyte chemotaxis. Overexpression can suppress the growth/viability of normal cells but not transformed cells (By similarity).
Species

Human
Source

HEK293
Tag

C-His
Accession

NP_037504 (L25-D184)
Gene ID

26585
Molecular Construction
N-term
(L25-D184)
Accession # NP_037504
His
C-term
Protein Length

Full Length of Mature Protein
Synonyms
GREM1,CKTSF1B1,DAND2,DRM,PIG2
Predicted Molecular Mass
20.2 kDa
Molecular Weight

Approximately 28-35 kDa,based on SDS-PAGE under reducing conditions,due to the glycosylation.
Glycosylation
Yes
Purity

Greater than 95% as determined by reducing SDS-PAGE.
Endotoxin Level

<1 EU/μg, determined by LAL method.
Documentation
SDS (251 KB)

COA (220 KB)

Handling Instructions (2659 KB)

Gremlin-1 Protein, Human (HEK293, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Gremlin-1 Protein, Human (HEK293, His)
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