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  4. GNAI2 Protein, Mouse (His)

GNAI2 Protein, Mouse (His) is the recombinant mouse-derived GNAI2 protein, expressed by E. coli, with N-6*His tag.

For research use only. We do not sell to patients.

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Description

GNAI2 Protein, Mouse (His) is the recombinant mouse-derived GNAI2 protein, expressed by E. coli, with N-6*His tag.
Background

GNAI2 is a Guanine nucleotide-binding protein (G protein) that acts as a modulator or transducer in various transmembrane signaling systems. As a member of the G(i) protein family, it inhibits adenylate cyclase in response to beta-adrenergic stimuli, participating in hormonal regulation. This protein may also play a role in cell division. by similar: Other G protein family members like GNAS are involved in transmembrane signaling but through distinct regulatory mechanisms.
Species

Mouse
Source

E. coli
Tag

N-6*His
Accession

P08752 (G2-F355)
Gene ID

14678
Molecular Construction
N-term
6*His
P08752 (G2-F355)
Accession # GNAI2
C-term
Protein Length

Full Length of Mature Protein
Synonyms
Guanine nucleotide-binding protein G(i) subunit alpha-2; Adenylate cyclase-inhibiting G alpha protein; GNAI2
AA Sequence

GCTVSAEDKAAAERSKMIDKNLREDGEKAAREVKLLLLGAGESGKSTIVKQMKIIHEDGYSEEECRQYRAVVYSNTIQSIMAIVKAMGNLQIDFADPQRADDARQLFALSCAAEEQGMLPEDLSGVIRRLWADHGVQACFGRSREYQLNDSAAYYLNDLERIAQSDYIPTQQDVLRTRVKTTGIVETHFTFKDLHFKMFDVGGQRSERKKWIHCFEGVTAIIFCVALSAYDLVLAEDEEMNRMHESMKLFDSICNNKWFTDTSIILFLNKKDLFEEKITQSSLTICFPEYTGANKYDEAASYIQSKFEDLNKRKDTKEIYTHFTCATDTKNVQFVFDAVTDVIIKNNLKDCGLF

Predicted Molecular Mass
44 kDa
Molecular Weight

Approximately 44 kDa,based on SDS-PAGE under reducing conditions.
Purity

Greater than 90% as determined by reducing SDS-PAGE.
Endotoxin Level

<1 EU/μg, determined by LAL method.
Documentation
Handling Instructions (2659 KB)

GNAI2 Protein, Mouse (His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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