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  4. Glypican-1/GPC1 Protein, Human (S486A, S488A, S490A, HEK293, His)

Glypican-1/GPC1 Protein, Human (S486A, S488A, S490A, HEK293, His)

Cat. No.: HY-P704638
Handling Instructions Technical Support

Glypican-1 (GPC1) is a heparan sulfate-containing cell surface proteoglycan. Glypican-1/GPC1 Protein, Human (S486A, S488A, S490A, HEK293, His) is the recombinant human-derived Glypican-1/GPC1 protein, expressed by HEK293, with C-His labeled tag.

For research use only. We do not sell to patients.

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Description

Glypican-1 (GPC1) is a heparan sulfate-containing cell surface proteoglycan. Glypican-1/GPC1 Protein, Human (S486A, S488A, S490A, HEK293, His) is the recombinant human-derived Glypican-1/GPC1 protein, expressed by HEK293, with C-His labeled tag.
Background

Glypican-1 (GPC1) Protein is a cell surface proteoglycan characterized by its association with heparan sulfate. It binds alpha-4 (V) collagen through the heparan sulfate side chains and plays a role in Schwann cell myelination. Additionally, GPC1 may act as a catalyst in the conversion of prion protein PRPN(C) to PRNP(Sc) by associating with both forms of PRPN, targeting them to lipid rafts, and facilitating their interaction. Moreover, GPC1 is essential for proper skeletal muscle differentiation, as it sequesters FGF2 in lipid rafts, preventing its binding to receptors (FGFRs) and inhibiting FGF-mediated signaling.
Species

Human
Source

HEK293
Tag

C-His
Accession

P35052-1 (D24-S530, S486A, S488A, S490A)
Gene ID

2817
Molecular Construction
N-term
GPC1 (D24-S530, S486A, S488A, S490A)
Accession # P35052-1
His
C-term
Synonyms
rHuGlypican-1/GPC1, His; Glypican-1; GPC1
Predicted Molecular Mass
57.9 kDa.
Molecular Weight

Approximately 58-61 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.
Glycosylation
Yes
Purity

Greater than 95% as determined by reducing SDS-PAGE.
Endotoxin Level

<1 EU/μg, determined by LAL method.
Documentation
Handling Instructions (2659 KB)

Glypican-1/GPC1 Protein, Human (S486A, S488A, S490A, HEK293, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Glypican-1/GPC1 Protein, Human (S486A, S488A, S490A, HEK293, His)
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