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  3. GLUT1 Protein, Human (sf9, His, N45T)

GLUT1 Protein, Human (sf9, His, N45T) is the recombinant human-derived GLUT1, expressed by sf9 insect cells , with His labeled tag. ,

For research use only. We do not sell to patients.

Surface Plasmon Resonance (SPR) Assay Service   Protein Expression Service

Size Price Stock Quantity
10 μg Get quote 3 - 4 weeks 2 - 3 weeks 2 - 3 weeks
50 μg Get quote 3 - 4 weeks 2 - 3 weeks 2 - 3 weeks
100 μg   Get quote  
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  • Biological Activity

  • Technical Parameters

  • Properties

  • Documentation

Description

GLUT1 Protein, Human (sf9, His, N45T) is the recombinant human-derived GLUT1, expressed by sf9 insect cells , with His labeled tag. ,

Background

Glucose Transporter GLUT1 is a facilitative glucose transporter responsible for constitutive or basal glucose uptake. It exhibits broad substrate specificity, capable of transporting various aldoses, including both pentoses and hexoses. As the primary energy carrier in the brain, GLUT1 is present at the blood-brain barrier and mediates energy-independent, facilitative glucose transport into the brain. In association with BSG and NXNL1, it promotes retinal cone survival by enhancing glucose uptake in photoreceptors (by similar). Additionally, it is required for mesendoderm differentiation (by similar).

Species

Human

Source

Sf9 insect cells

Tag

His

Accession

P11166 (M1-V492, N45T)

Gene ID

6513

Protein Length

Full Length

Synonyms
GLUT1
Predicted Molecular Mass
55.4 kDa
Glycosylation
Yes
Purity

Greater than 95% as determined by reducing SDS-PAGE

Appearance

Solution.

Formulation

Supplied as a 0.2 μm filtered solution of 25 mM Tris-HCl pH 8.0, 150 mM NaCl, 0.002%, w/v LMNG.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

Please use rapid thawing with running water to thaw the protein.

Storage & Stability

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

Documentation

GLUT1 Protein, Human (sf9, His, N45T) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
GLUT1 Protein, Human (sf9, His, N45T)
Cat. No.:
HY-P703198
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