2. Recombinant Proteins
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  4. GFP Protein, Bacteria (His)

GFP Protein, Bacteria (His) is the recombinant GFP, expressed by E. coli , with His labeled tag.

For research use only. We do not sell to patients.

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Description

GFP Protein, Bacteria (His) is the recombinant GFP, expressed by E. coli , with His labeled tag.
Background

The Green Fluorescent Protein (GFP) serves as an energy-transfer acceptor, converting the blue chemiluminescence emitted by the protein aequorin into green fluorescent light through energy transfer. GFP's unique ability to fluoresce in vivo is contingent upon receiving energy from the Ca(2+)-activated photoprotein aequorin. This remarkable property has made GFP an invaluable tool in molecular and cellular biology, enabling the visualization of specific proteins and cellular structures in living organisms. The utilization of GFP as a fluorescent marker has revolutionized biological research, facilitating non-invasive and real-time imaging of dynamic cellular processes (
Species

Others
Source

E. coli
Tag

His
Accession

NC_011521.1 (M1-K238)
Gene ID

7011691
Protein Length

Partial
Synonyms
gfp
Predicted Molecular Mass
39 kDa
Purity

Greater than 80% as determined by reducing SDS-PAGE
Documentation
Handling Instructions (2659 KB)

GFP Protein, Bacteria (His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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GFP Protein, Bacteria (His)
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HY-P703060
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