GALNT7 Protein, Human (HEK293, His)

The GALNT7 protein serves as an indispensable glycopeptidyl transferase for O-linked oligosaccharide biosynthesis. Its enzymatic activity involves catalyzing the transfer of N-acetyl-D-galactosamine residues to pre-existing glycosylated peptides. GALNT7 Protein, Human (HEK293, His) is the recombinant human-derived GALNT7 protein, expressed by HEK293 , with C-6*His labeled tag.

GALNT7 is a glycopeptide transferase that plays a crucial role in O-linked oligosaccharide biosynthesis. Unlike other members of its protein family, GALNT7 does not act as a peptide transferase that directly transfers N-acetyl-D-galactosamine (GalNAc) onto a serine or threonine residue on the protein receptor. Instead, it catalyzes the transfer of GalNAc to an already glycosylated peptide, requiring the prior addition of a GalNAc on a peptide before adding additional GalNAc moieties. While GALNT7 primarily functions in O-linked glycosylation, some peptide transferase activity cannot be entirely excluded, and the identification of its appropriate peptide substrate remains a subject of ongoing investigation.

Measured by its ability to transfer galactose from UDP-GalNAc (HY-114365) to peptide MUC5AC-3/13 that incubate at 37℃ for 20 min. The specific activity is 1288.83 pmol/min/μg.

Human

HEK293

C-6*His

Q86SF2 (P30-V657)

51809  [NCBI]

Lumenal Domain

PRPDDPSPLSRMREDRDVNDPMPNRGGNGLAPGEDRFKPVVPWPHVEGVEVDLESIRRINKAKNEQEHHAGGDSQKDIMQRQYLTFKPQTFTYHDPVLRPGILGNFEPKEPEPPGVVGGPGEKAKPLVLGPEFKQAIQASIKEFGFNMVASDMISLDRSVNDLRQEECKYWHYDENLLTSSVVIVFHNEGWSTLMRTVHSVIKRTPRKYLAEIVLIDDFSNKEHLKEKLDEYIKLWNGLVKVFRNERREGLIQARSIGAQKAKLGQVLIYLDAHCEVAVNWYAPLVAPISKDRTICTVPLIDVINGNTYEIIPQGGGDEDGYARGAWDWSMLWKRVPLTPQEKRLRKTKTEPYRSPAMAGGLFAIEREFFFELGLYDPGLQIWGGENFEISYKIWQCGGKLLFVPCSRVGHIYRLEGWQGNPPPIYVGSSPTLKNYVRVVEVWWDEYKDYFYASRPESQALPYGDISELKKFREDHNCKSFKWFMEEIAYDITSHYPLPPKNVDWGEIRGFETAYCIDSMGKTNGGFVELGPCHRMGGNQLFRINEANQLMQYDQCLTKGADGSKVMITHCNLNEFKEWQYFKNLHRFTHIPSGKCLDRSEVLHQVFISNCDSSKTTQKWEMNNIHSV

65-80 kDa

Greater than 95% as determined by reducing SDS-PAGE.

Solution.

Supplied as a 0.22 μm filtered solution of 50 mM Tris-HCl, 10 mM reduced Glutathione, pH 8.0.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.