Galectin-8/LGALS8 Protein, Rat (His)

Galectin-8 belongs to the family of animal lectins that bind to different glycoconjugates. Galectin-8 inhibits MTOR and AMPK signaling pathways to induce autophagy in response to lysosomal damage. Galectin-8 is highly expressed in a variety of tumors. Galectin-8 Protein, Rat (His) is the recombinant rat-derived Galectin-8, expressed by E. coli, with N-6*His labeled tag. The total length of Galectin-8 Protein, Rat (His) is 316 a.a..

Galectin-8 belongs to the family of animal lectins that bind to different glycoconjugates. As a secreted protein, Galectin-8 is found in body fluids or the serum of patients with breast cancer and those with colon cancer. Extracellular Galectin-8 promotes cell adhesion by binding to cell adhesion molecules such as integrins CD44, CD166 and Podoplanin. Galectin-8 performs its intracellular function by marking the vacuoles invaded by pathogens to be destroyed by autophagy. Galectin-8 inhibited MTOR and AMPK in response to lysosomal injury induced autophagy in membrane disturbances. Galectin-8 is highly expressed in a variety of tumors^[1][2][3].

Measured by its binding ability in a functional ELISA. Immobilized SLC31A1 at 5 μg/mL (100 μL/well) can bind Rat Galectin-8. The ED₅₀ for this effect is 3.773 μg/mL.

• 
  Measured by its binding ability in a functional ELISA. Immobilized SLC31A1 at 5 μg/mL (100 μL/well) can bind Rat Galectin-8, The ED₅₀ for this effect is 3.773 μg/mL.

Rat

E. coli

N-6*His

Q6IN24 (M1-W316)

116641

Full Length

MLSLSNLQNIIYNPTIPYVSTITEQLKPGSLIVIRGHVPKDSERFQVDFQHGNSLKPRADVAFHFNPRFKRSNCIVCNTLTNEKWGWEEITHDMPFRKEKSFEIVIMVLKNKFQVAVNGKHILLYAHRINPEKIDTLGIYGKVNIHSIGFRFSSDLQSMETSTLGLTQISKENIQKSGKLHLSLPFEARLNASMGPGRTVVVKGEVNTNAKSFNVDLVAGRSRDIALHLNPRLNVKAFVRNSFLQDAWGEEERNITCFPFSSGMYFEMIIYCDVREFKVAVNGVHSLEYKHRFKDLSSIDTLAVDGDIRLLDVRSW

Approximately 34 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 10% glycerol.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Zick Y. Galectin-8, cytokines, and the storm. Biochem Soc Trans. 2022 Feb 28;50(1):135-149.  [Content Brief]

  [2]. Storti P, et al. Role of Galectins in Multiple Myeloma. Int J Mol Sci. 2017 Dec 17;18(12):2740.  [Content Brief]

  [3]. Jia J, et al. Galectins control MTOR and AMPK in response to lysosomal damage to induce autophagy. Autophagy. 2019 Jan;15(1):169-171.  [Content Brief]