G3BP1 Protein, Human (466a.a, His)

G3BP1 Protein, Human (His) is the recombinant human-derived G3BP1, expressed by E. coli , with N-6*His labeled tag.

G3BP is a multifunctional protein involved in processes such as stress granule formation and innate immunity. It plays an essential role in stress granule formation, which are membraneless compartments storing mRNAs and proteins during stress responses. G3BP promotes stress granule assembly through liquid-liquid phase separation (LLPS), acting as a molecular switch that triggers RNA-dependent LLPS in response to increased intracellular free RNA concentrations. Additionally, G3BP exhibits ATP- and magnesium-dependent helicase activity, unwinding DNA/DNA, RNA/DNA, and RNA/RNA substrates with comparable efficiency while moving unidirectionally along single-stranded DNA. In innate immunity, G3BP enhances CGAS and RIGI activity, promoting the DNA-triggered cGAS/STING pathway and RIGI-mediated type I interferon production. In vitro, G3BP may also function as a phosphorylation-dependent sequence-specific endoribonuclease, preferentially cleaving MYC mRNA at the 3'-UTR. by similar: G3BP is implicated in cGAS condensation, likely associated with the formation of membrane-less organelles.

Measured in a cell proliferation assay using HCT116 cells. The ED₅₀ for this effect is 5.264 ng/mL, corresponding to a specific activity is 1.9×10^5 units/mg.

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  Measured in a cell proliferation assay using HCT116 cells. The ED₅₀ for this effect is 5.264 ng/mL, corresponding to a specific activity is 1.9×10^5 units/mg.

Human

E. coli

N-6*His

Q13283-1 (M1-Q466)

10146

Full Length of Isoform-1

Approximately 65-69 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 6% or 8% Trehalose.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.