G-CSFR/CD114 Protein, Mouse (HEK293, His)

The G-CSFR/CD114 protein serves as a receptor for granulocyte colony-stimulating factor (CSF3) and plays a key role in granulocyte development and function. As a homodimer, it binds two CSF3 molecules and transduces signals. G-CSFR/CD114 Protein, Mouse (HEK293, His) is the recombinant mouse-derived G-CSFR/CD114 protein, expressed by HEK293 , with C-His labeled tag.

G-CSFR/CD114 Protein acts as the receptor for granulocyte colony-stimulating factor (CSF3) and may additionally play a role in certain adhesion or recognition events at the cell surface. Existing as a homodimer, the receptor binds two CSF3 molecules, facilitating the transduction of signals related to granulocyte development and function. Moreover, G-CSFR/CD114 interacts with CEACAM1, leading to the down-regulation of the CSF3R-STAT3 pathway. This process involves the recruitment of PTPN6, which subsequently dephosphorylates CSF3R, revealing a regulatory aspect of G-CSFR/CD114 in modulating signaling pathways associated with granulocyte colony-stimulating factor responses. These interactions highlight the intricate regulatory roles of G-CSFR/CD114 in cellular processes related to immune responses and hematopoiesis.

Immobilized Mouse G-CSF R, His Tag at 0.2 μg/mL (100 μl/well) on the plate. Dose response curve for Human G-CSF, hFc Tag with the EC₅₀ of ≤3 ng/mL determined by ELISA.

Mouse

HEK293

C-8*His

P40223 (C26-D626)

12986  [NCBI]

Extracellular Domain

90-110 kDa

Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 8% trehalose.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.