FUS Protein, Human (MBP-His)

FUS Protein, Human (MBP-His) is the recombinant human-derived FUS protein, expressed by E. coli, with N-His & N-MBP tag.

TLS/FUS is a DNA/RNA-binding protein involved in multiple cellular processes including transcription regulation, RNA splicing, RNA transport, DNA repair, and damage response. It specifically binds single-stranded RNA containing the 5'-AGGUAA-3' consensus sequence and interacts with nascent pre-mRNAs, serving as a molecular bridge between RNA polymerase II and U1 small nuclear ribonucleoprotein to couple transcription with splicing. The protein autoregulates its expression by binding its own pre-mRNA via nonsense-mediated decay. During DNA double-strand break repair, it promotes D-loop formation and homologous recombination. In neuronal cells (by similar), it plays essential roles in dendritic spine formation/stability, RNA transport, mRNA stability, and synaptic homeostasis.

Human

E. coli

N-His;N-MBP

P35637-1 (M1-Y526)

2521

Approximately 95 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

Greater than 85% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.