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  4. FLI1 Protein, Human

FLI1 Protein, Human is the recombinant human-derived FLI1, expressed by E. coli , with tag Free labeled tag.

For research use only. We do not sell to patients.

Surface Plasmon Resonance (SPR) Assay Service   Protein Expression Service

Size Price Stock Quantity
10 μg Get quote 3 - 4 weeks 2 - 3 weeks 2 - 3 weeks
50 μg Get quote 3 - 4 weeks 2 - 3 weeks 2 - 3 weeks
100 μg   Get quote  
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Top Publications Citing Use of Products

  • Biological Activity

  • Technical Parameters

  • Properties

  • Documentation

Description

FLI1 Protein, Human is the recombinant human-derived FLI1, expressed by E. coli , with tag Free labeled tag.
Background

FLI1 is a sequence-specific transcriptional activator that recognizes the DNA sequence 5'-C[CA]GGAAGT-3'. Its function has been demonstrated in multiple studies, including gene expression regulation. By similar research, FLI1 plays a significant role in transcriptional regulation.
Species

Human
Source

E. coli
Tag

Tag Free
Accession

Q01543 (P276-N399)
Gene ID

2313
Protein Length

Partial
Synonyms
Friend leukemia integration 1 transcription factor; Proto-oncogene Fli-1; Transcription factor ERGB; FLI1; Homo sapiens; Human; Activator; DNA-binding
Predicted Molecular Mass
14.7 kDa
Purity

Greater than 95% as determined by reducing SDS-PAGE
Appearance

Solution.
Formulation

Supplied as a 0.22 μm filtered solution of 50 mM HEPES (pH 7.5), 200 mM NaCl, 20% glycerol, 1 mM DTT.
Endotoxin Level

<1 EU/μg, determined by LAL method.
Reconstitution

Please use rapid thawing with running water to thaw the protein.
Storage & Stability

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping

Shipping with dry ice.
Documentation
Handling Instructions (2659 KB)

FLI1 Protein, Human Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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FLI1 Protein, Human
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HY-P703126
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