FGFR-2 Protein, Mouse (HEK293, His)

FGFR-2 Protein, Mouse (HEK293, His) is the recombinant mouse-derived FGFR-2, expressed by HEK293 , with C-6*His labeled tag.

FGFR2, also known as CD332 (differentiated cluster 332), is a receptor for fibroblast growth factor. FGFR2 plays an important role in embryonic development and tissue repair, especially bone and blood vessels. It promotes the proliferation of keratinocytes and immature osteoblasts and promotes the apoptosis of differentiated osteoblasts. After ligand binding, FGFR-2 α IIIc activates several signaling cascades, including phosphorylation of PLCG1, FRS2, and PAK4. Activation of PLCG1 triggers the production of cell signaling molecules such as diacylglycerol and inositol 1,4, 5-triphosphate. FRS2 phosphorylation leads to recruitment of GRB2, GAB1, PIK3R1, and SOS1, mediating activation of RAS, MAPK1/ERK2, MAPK3/ERK1, MAP kinase signaling pathways, and AKT1 signaling pathways. FGFR2 up-regulates PAI-1 via JAK2/STAT3 signaling and induces M2 polarization in colorectal cancer macrophages^[1][2][3].

Measured by its ability to inhibit FGF-acidic dependent proliferation of Balb/c 3T3 mouse fibroblasts. The ED₅₀ for this effect is 1.667 ng/mL, corresponding to a specific activity is 6.0×10⁵ units/mg.

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  Measured by its ability to inhibit FGF-acidic dependent proliferation of Balb/c 3T3 mouse fibroblasts. The ED₅₀ for this effect is 1.667 ng/mL, corresponding to a specific activity is 6.0×10⁵ units/mg.

Mouse

HEK293

C-6*His

E9Q5C3/AAA39377.1 (R22-E263)

14183  [NCBI]

Partial

RPSFSLVEDTTLEPEGAPYWTNTEKMEKRLHAVPAANTVKFRCPAGGNPTPTMRWLKNGKEFKQEHRIGGYKVRNQHWSLIMESVVPSDKGNYTCLVENEYGSINHTYHLDVVERSPHRPILQAGLPANASTVVGGDVEFVCKVYSDAQPHIQWIKHVEKNGSKYGPDGLPYLKVLKHSGINSSNAEVLALFNVTEMDAGEYICKVSNYIGQANQSAWLTVLPKQQAPVREKEITASPDYLE

Approximately 40-7 kDa due to the glycosylation.

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  Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.