FGF basic/bFGF Protein, Human (Biotinylated, HEK293, His-Avi)

FGF basic, or bFGF (fibroblast growth factor basic), initiates at an alternative CUG codon, marking a distinctive feature in translational initiation. This alternative start codon plays a pivotal role in regulating the expression and functional properties of FGF basic. FGF basic/bFGF Protein, Human (Biotinylated, HEK293, His-Avi) is the recombinant human-derived FGF basic/bFGF protein, expressed by HEK293 , with N-His, N-Avi labeled tag. The total length of FGF basic/bFGF Protein, Human (Biotinylated, HEK293, His-Avi) is 146 a.a., with molecular weight of 20-22 kDa.

Initiation of FGF basic, also known as bFGF (fibroblast growth factor basic), occurs at an alternative CUG codon. This alternative start codon represents a distinctive feature in the translational initiation of the protein, contributing to the unique regulatory mechanisms governing the expression and functional properties of FGF basic.

Immobilized Human Glypican-3 Fc at 5 μg/mL (100 μL/well) can bind Biotinylated Human FGF basic Avi-His with a linear range of 0.6-10 ng/mL.

Human

HEK293

N-His;N-Avi

P09038-4 (P143-S288)

2247  [NCBI]

20-22 kDa

Greater than 90% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized a 0.22 μm filtered solution of 50 mM Tris,150 mM NaCl,pH 7.5 . Normally trehalose is added as protectant before lyophilization.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.