FcgR4/CD16-2 Protein, Mouse (HEK293, His)

The FcγR4/CD16-2 protein is an IgG receptor with moderate affinity for IgG2a and IgG2b. It recognizes virus-specific IgG, triggers antibody-dependent cellular cytotoxicity, and provides protection against fatal influenza infection. FcgR4/CD16-2 Protein, Mouse (HEK293, His) is the recombinant mouse-derived FcgR4/CD16-2 protein, expressed by HEK293 , with C-His labeled tag.

The FcγR4/CD16-2 Protein serves as a receptor for the constant Fc fragment of immunoglobulin gamma (IgG), exhibiting intermediate affinity for both IgG2a and IgG2b. It recognizes neutralizing virus-specific IgGs on infected cells, triggering antibody-dependent cellular cytotoxicity (ADCC) and conferring protection against lethal influenza virus infection. On splenic dendritic cells, FcγR4 efficiently uptakes antigen immune complexes, directing them into MHC class I and II antigen presentation pathways, thereby enhancing CD4-positive and CD8-positive T cell immune responses. Additionally, FcγR4 plays a crucial role in mediating neutrophil activation by IgG complexes, acting redundantly with FCGR2A. It contributes to bone resorption by enhancing osteoclast differentiation upon binding to IgG2a. Furthermore, FcγR4 functions as a receptor for the Fc region of immunoglobulin epsilon (IgE), binding to both a and b allotypes of IgE and promoting macrophage-mediated phagocytosis, antigen presentation to T cells, and the late phase of cutaneous allergic reactions. It forms a heterooligomeric complex with ITAM-containing signaling subunits FCER1G and interacts with the Fc region of antigen-complexed IgG.

Measured by its binding ability in a functional ELISA.Immobilized mouse FCGR4 at 10 μg/mL (100 μl/well) can bind recombinant human IgG1 (Fc) . The EC₅₀ of human IgG1 (Fc) is 0.11-0.25 μg/mL.

Mouse

HEK293

C-His

A0A0B4J1G0 (Q19-Q203)

246256  [NCBI]

Partial

25-35 kDa

Greater than 95% as determined by reducing SDS-PAGE

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.