Enterokinase Protein, Bovine (P.pastoris, His)

Enterokinase Protein, Bovine (P.pastoris, His) is a glycosylated peptide chain with a His-tag produced in P. pastoris. Enterokinase Protein is a serine protease that plays a crucial role in the digestive system. By specifically cleaving trypsinogen to convert it into active trypsin, Enterokinase Protein initiates the cascade activation of pancreatic zymogens. Enterokinase Protein serves as a key initiator of the intestinal digestive enzyme cascade^[1][2][3].

Enterokinase is a glycoprotein mainly present in the duodenal and jejunal mucosa. The core role of enterokinase is to activate digestive zymogens, converting trypsinogen into trypsin. The activated trypsin can not only directly decompose proteins but also further activate other zymogens such as chymotrypsinogen, procarboxypeptidase, and proelastase, forming a digestive enzyme cascade reaction to promote protein digestion^[1][2][3].

100 IU/μg Unit Definition: One unit is defined as the amount of enzyme needed to cleave 50 μg of fusion protein in 16 hours to 95% completion at 22°C in a buffer containing 25mM Tris-HCl, pH 8.0.

Bovine

P. pastoris

C-His

/

/

Approximately 40 kDa

Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized after extensive dialysis against 20 mM Tris-HCl, pH 7.4, 200 mM NaCl, 2 mM CaCl₂.

<0.2 EU/μg, determined by LAL method.

Reconstitute at 1 mg/mL in sterile deionied water, then dilute in sterile EK Storage Buffer (20 mM Tris-HCl, pH 7.4, 200 mM NaCl, 2 mM CaCl₂).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Light A, et al. The amino-terminal sequence of the catalytic subunit of bovine enterokinase. J Protein Chem. 1991 Oct;10(5):475-80.  [Content Brief]

  [2]. Mann NS, et al. Enterokinase. Proc Soc Exp Biol Med. 1994 Jun;206(2):114-8.  [Content Brief]

  [3]. Kitamoto Y, et al. Enterokinase, the initiator of intestinal digestion, is a mosaic protease composed of a distinctive assortment of domains. Proc Natl Acad Sci U S A. 1994 Aug 2;91(16):7588-92.  [Content Brief]