1. Recombinant Proteins
  2. Cytokines and Growth Factors
  3. Endothelin-3/EDN3 Protein, Human (P. pastoris, His, Myc)

Endothelin-3/EDN3 Protein, Human (P. pastoris, His, Myc)

Cat. No.: HY-P704078
Handling Instructions Technical Support

Endothelin-3/EDN3 Protein, Human (P. pastoris, His, Myc) is the recombinant human-derived Endothelin-3/EDN3 protein, expressed by P. pastoris, with C-6*His & C-Myc tag.

For research use only. We do not sell to patients.

Surface Plasmon Resonance (SPR) Assay Service   Protein Expression Service

Size Price Stock Quantity
20 μg In-stock
50 μg In-stock
100 μg   Get quote  

* Please select Quantity before adding items.

Top Publications Citing Use of Products
  • Biological Activity

  • Technical Parameters

  • Properties

  • Documentation

Description

Endothelin-3/EDN3 Protein, Human (P. pastoris, His, Myc) is the recombinant human-derived Endothelin-3/EDN3 protein, expressed by P. pastoris, with C-6*His & C-Myc tag.

Background

Endothelin-3 (EDN3) is an endothelium-derived vasoconstrictor peptide belonging to the endothelin family. by similar: Other endothelin family members such as Endothelin-1 and Endothelin-2 share similar vasoconstrictive properties.

Species

Human

Source

P. pastoris

Tag

C-6*His;C-Myc

Accession

P14138 (C97-W117)

Gene ID

1908

Molecular Construction
N-term
EDN3 (C97-W117)
Accession # P14138
6*His
Myc
C-term
Protein Length

Full Length of Mature Peptide

Synonyms
ET-3; Preproendothelin-3; PPET3
AA Sequence

CTCFTYKDKECVYYCHLDIIW

Molecular Weight

Approximately 8 kDa, based on SDS-PAGE under reducing conditions.

Purity

Greater than 85% as determined by reducing SDS-PAGE.

Appearance

Lyophilized powder

Formulation

Lyophilized from a 0.2 μm filtered solution of 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation

Endothelin-3/EDN3 Protein, Human (P. pastoris, His, Myc) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

Your Recently Viewed Products:

Inquiry Online

Your information is safe with us. * Required Fields.

Product Name

 

Requested Quantity *

Applicant Name *

 

Salutation

Email Address *

 

Phone Number *

Department

 

Organization Name *

City

State

Country or Region *

     

Remarks

Bulk Inquiry

Inquiry Information

Product Name:
Endothelin-3/EDN3 Protein, Human (P. pastoris, His, Myc)
Cat. No.:
HY-P704078
Quantity:
MCE Japan Authorized Agent: