EMAP-II Protein, Human (His)

EMAP-II Protein, Human (His) is the recombinant human-derived EMAP-II protein, expressed by E. coli, with N-His tag.

EMAP-II serves as a non-catalytic component within the multisynthase complex (MSC), playing a crucial role in orchestrating the functions of various tRNA ligases. It specifically stimulates the catalytic activity of cytoplasmic arginyl-tRNA synthase and binds to tRNA. Apart from its role in protein synthesis, EMAP-II exhibits inflammatory cytokine activity and negatively regulates TGF-beta signaling by stabilizing SMURF2 and inhibiting its SMAD7-mediated degradation. Additionally, EMAP-II contributes to glucose homeostasis through the induction of glucagon secretion, promotes dermal fibroblast proliferation and wound repair, and modulates endothelial cell responses by inducing migration at low concentrations and apoptosis at high concentrations. It forms homodimers and is part of the MSC, interacting with various tRNA ligases, SMURF2, HSP90B1/gp96, and PSMA7, showcasing its involvement in diverse cellular processes and regulatory networks.

Human

E. coli

N-His

Q12904-1 (A2-K312)

9255

Full Length of Isoform-1 Mature Protein

ANNDAVLKRLEQKGAEADQIIEYLKQQVSLLKEKAILQATLREEKKLRVENAKLKKEIEELKQELIQAEIQNGVKQIPFPSGTPLHANSMVSENVIQSTAVTTVSSGTKEQIKGGTGDEKKAKEKIEKKGEKKEKKQQSIAGSADSKPIDVSRLDLRIGCIITARKHPDADSLYVEEVDVGEIAPRTVVSGLVNHVPLEQMQNRMVILLCNLKPAKMRGVLSQAMVMCASSPEKIEILAPPNGSVPGDRITFDAFPGEPDKELNPKKKIWEQIQPDLHTNDECVATYKGVPFEVKGKGVCRAQTMSNSGIK

Approximately 40-45 kDa,based on SDS-PAGE under reducing conditions.

Greater than 95% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.