EGFR Protein, Human (sf9, His-GST)

EGFR is a transmembrane glycoprotein. EGFR binds to ligands, triggering receptor dimerization, inducing intracellular TK domain autophosphorylation, activating downstream Ras/MAPK, PI3K/Akt and other signaling pathways, and regulating cell proliferation, survival, migration and angiogenesis. EGFR Protein, Human (sf9, His-GST) is a recombinant EGFR protein expressed by Sf9 insect cells with N-His, N-GST tags.

EGFR belongs to the epidermal growth factor receptor family^{[1][2][3][4][5]}. EGFR is a transmembrane glycoprotein. EGFR has an extracellular domain of 621 amino acids, a single transmembrane domain of 23 amino acids, and an enzymatically active intracellular domain of 542 amino acids^[6]. EGFR binds to its ligand, triggers receptor dimerization, induces autophosphorylation of the intracellular TK domain, activates downstream Ras/MAPK, PI3K/Akt and other signaling pathways, and regulates cell proliferation, survival, migration and angiogenesis^[1][3][4][5]. EGFR is expressed in a variety of normal epithelial tissues and tumor tissues, such as breast, lung, stomach, colon, head and neck, and its abnormal expression is associated with the malignant progression of tumors^{[1][2][3][4][5]}.

The specific activity was determined to be ≥4 nmol/min/mg using Poly(Glu:Tyr) 4:1 as substrate.

Human

Sf9 insect cells

N-His;N-GST

P00533-1 (M668-A1210)

1956  [NCBI]

Cytoplasmic Domain

MRRRHIVRKRTLRRLLQERELVEPLTPSGEAPNQALLRILKETEFKKIKVLGSGAFGTVYKGLWIPEGEKVKIPVAIKELREATSPKANKEILDEAYVMASVDNPHVCRLLGICLTSTVQLITQLMPFGCLLDYVREHKDNIGSQYLLNWCVQIAKGMNYLEDRRLVHRDLAARNVLVKTPQHVKITDFGLAKLLGAEEKEYHAEGGKVPIKWMALESILHRIYTHQSDVWSYGVTVWELMTFGSKPYDGIPASEISSILEKGERLPQPPICTIDVYMIMVKCWMIDADSRPKFRELIIEFSKMARDPQRYLVIQGDERMHLPSPTDSNFYRALMDEEDMDDVVDADEYLIPQQGFFSSPSTSRTPLLSSLSATSNNSTVACIDRNGLQSCPIKEDSFLQRYSSDPTGALTEDSIDDTFLPVPEYINQSVPKRPAGSVQNPVYHNQPLNPAPSRDPHYQDPHSTAVGNPEYLNTVQPTCVNSTFDSPAHWAQKGSHQISLDNPDYQQDFFPKEAKPNGIFKGSTAENAEYLRVAPQSSEFIGA

Approximately 89.1 kDa

Greater than 80% as determined by reducing SDS-PAGE

Solution

Supplied as a 0.2 μm filtered solution of 20 mM Tris, 500 mM NaCl, 10 % glycerol, pH 7.4 or 20 mM Tris, 300 mM NaCl, 10% Glycerol, pH 7.5, 0.02% Triton X-100, 0.4 mM β-mercaptoethanol, 0.5 mM PMSF, 2 mM GSH.

<1 EU/μg, determined by LAL method.

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.

• [1]. Lemmon M A, et al. Cell signaling by receptor tyrosine kinases. Cell, 2010, 141(7): 1117-1134.

  [2]. Giaccone G. HER1/EGFR-targeted agents: predicting the future for patients with unpredictable outcomes to therapy. Ann Oncol. 2005 Apr;16(4):538-48.  [Content Brief]

  [3]. Yarden Y, et al. Untangling the ErbB signalling network. Nat Rev Mol Cell Biol. 2001 Feb;2(2):127-37.  [Content Brief]

  [4]. Shepard HM, et al. Signal integration: a framework for understanding the efficacy of therapeutics targeting the human EGFR family. J Clin Invest. 2008 Nov;118(11):3574-81.  [Content Brief]

  [5]. Okines A, et al. Targeting the human EGFR family in esophagogastric cancer. Nat Rev Clin Oncol. 2011 Apr 5;8(8):492-503.  [Content Brief]

  [6]. Ho ECH, et al. Characterization of monoclonal antibodies generated to the 287-302 amino acid loop of the human epidermal growth factor receptor'. Antib Ther. 2019 Oct;2(4):88-98.  [Content Brief]