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  4. DNAJC9 Protein, Human (His, Strep)

DNAJC9 Protein, Human (His, Strep) is the recombinant human-derived DNAJC9, expressed by E. coli , with Strep, His labeled tag.

For research use only. We do not sell to patients.

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Description

DNAJC9 Protein, Human (His, Strep) is the recombinant human-derived DNAJC9, expressed by E. coli , with Strep, His labeled tag.
Background

DNAJC9 functions as both a histone chaperone and a heat shock co-chaperone. As a histone chaperone, it forms a complex with MCM2 and histone H3-H4 heterodimers, aiding MCM2 in recognizing histone H3-H4 and facilitating histone assembly into nucleosomes. It may also act as a histone co-chaperone alongside TONSL and recruit histone chaperones such as ASF1A, NASP, and SPT2 to histone H3-H4 heterodimers. Additionally, DNAJC9 serves as a co-chaperone for HSP70 family molecular chaperones (e.g., HSPA1A, HSPA1B, and HSPA8), potentially playing a role in recruiting the HSP70 chaperone machinery to histone H3-H4 substrates to maintain histone structural integrity. In vitro, it demonstrates the ability to assemble histones onto DNA.
Species

Human
Source

E. coli
Tag

Strep;His
Accession

Q8WXX5 (G2-K260)
Gene ID

23234
Protein Length

Partial
Synonyms
DnaJ homolog subfamily C member 9; DnaJ protein SB73; DNAJC9; Homo sapiens; Human; Chaperone
Predicted Molecular Mass
33 kDa
Purity

Greater than 80% as determined by reducing SDS-PAGE
Endotoxin Level

<1 EU/μg, determined by LAL method.
Documentation
Handling Instructions (2659 KB)

DNAJC9 Protein, Human (His, Strep) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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