2. Recombinant Proteins
  3. Enzymes & Regulators
  4. Transferases (EC 2)
  5. DHHC20 Protein, Human (sf9)

DHHC20 Protein, Human (sf9) is the recombinant human-derived DHHC20, expressed by sf9 insect cells , with tag Free labeled tag.

For research use only. We do not sell to patients.

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  • Biological Activity

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Description

DHHC20 Protein, Human (sf9) is the recombinant human-derived DHHC20, expressed by sf9 insect cells , with tag Free labeled tag.
Background

DHHC20 is a palmitoyltransferase that catalyzes the addition of palmitate to various protein substrates. It mediates palmitoylation of Cys residues in the cytoplasmic C-terminus of EGFR, modulating the duration of EGFR signaling by regulating palmitoylation-dependent EGFR internalization and degradation. This enzyme exhibits a preference for acyl-CoA with C16 fatty acid chains but can also utilize acyl-CoA with C14 and C18 chains. Additionally, DHHC20 may palmitoylate the CALHM1 subunit of gustatory voltage-gated ion channels, influencing channel gating and kinetics. by similar
Species

Human
Source

Sf9 insect cells
Tag

Tag Free
Accession

Q5W0Z9-1 (M1-N365)
Gene ID

253832
Protein Length

Full Length of Isoform-1
Synonyms
Palmitoyltransferase ZDHHC20; Acyltransferase ZDHHC20; 2.3.1.-; DHHC domain-containing cysteine-rich protein 20; DHHC20; Zinc finger DHHC domain-containing protein 20; ZDHHC20; Homo sapiens; Human; Acyltransferase; Transferase; 2.3.1.225
Predicted Molecular Mass
42 kDa
Purity

Greater than 90% as determined by reducing SDS-PAGE
Endotoxin Level

<1 EU/μg, determined by LAL method.
Documentation
Handling Instructions (2659 KB)

DHHC20 Protein, Human (sf9) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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DHHC20 Protein, Human (sf9)
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